LS-C354966

antibody from LSBio

Targeting: BID

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C354966

Provider

LSBio

Product name

BID Antibody LS-C354966

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western Blot Extracts of Jurkat cells without added BID (lanes 1 and 2), with caspase-8 cleaved recombinant human BID (lane 3), or with caspase-8 cleaved recombinant mouse BID (lane 4) were resolved by SDS-PAGE on a 4-20% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature, then incubated with either a human BID full length antibody (lane 1) or the human BID (p15) cleavage site-specific antibody (lanes 2-4) for two hours at room temperature in a 1% BSA-TBST buffer. After washing, the membrane was incubated with goat F (ab')2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Pierce SuperSignal method. The data show that the human BID (p15) antibody recognizes only the human 15 kDa BID fragment, demonstrating the specificity of the antibody.