Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-C354966 - Provider product page
- Provider
- LSBio
- Product name
- BID Antibody LS-C354966
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
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- LSBio (provider)
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- Experimental details
- Western Blot Extracts of Jurkat cells without added BID (lanes 1 and 2), with caspase-8 cleaved recombinant human BID (lane 3), or with caspase-8 cleaved recombinant mouse BID (lane 4) were resolved by SDS-PAGE on a 4-20% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature, then incubated with either a human BID full length antibody (lane 1) or the human BID (p15) cleavage site-specific antibody (lanes 2-4) for two hours at room temperature in a 1% BSA-TBST buffer. After washing, the membrane was incubated with goat F (ab')2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Pierce SuperSignal method. The data show that the human BID (p15) antibody recognizes only the human 15 kDa BID fragment, demonstrating the specificity of the antibody.