TA506768

antibody from OriGene

Targeting: BID

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA506768

Provider

OriGene

Proper citation

OriGene Cat#TA506768, RRID:AB_2623893

Product name

BID mouse monoclonal antibody, clone OTI3C8 (formerly 3C8)

Antibody type

Monoclonal

Description

BID mouse monoclonal antibody, clone OTI3C8 (formerly 3C8)

Host

Mouse

Conjugate

Unconjugated

Epitope

BID

Isotype

IgG

Antibody clone number

OTI3C8

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BID (RC207261, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BID.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BID monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-BID mouse monoclonal antibody (TA506768) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BID(RC207261).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-BID mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506768)

Validation comment

IHC