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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [6]
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- Product number
- UM500101 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#UM500101, RRID:AB_2629111
- Product name
- BID mouse monoclonal antibody,clone UMAB139
- Antibody type
- Monoclonal
- Description
- BID mouse monoclonal antibody,clone UMAB139
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- BID
- Isotype
- IgG
- Antibody clone number
- UMAB139
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of DU145 cell lysate (35ug) by using anti-BID monoclonal antibody UM500101
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-BID monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-BID mouse monoclonal antibody. (UM500101, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-BID mouse monoclonal antibody. (UM500101, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded of 3 human colon cancers using anti-BID clone UMAB139 mouse monoclonal antibody at 1:200 dilution of 0.6mg/mL and detection with Polink2 Broad HRP DAB. UM500101 requires heat-induced epitope retrieval with citrate pH6.0 at 95-100C 20 minutes. The composit image of 3 colon cancers labeled A, B, and C shows strong membranous and cytoplasmic staining in >75 % of tumor cells and image A and B shows
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human kidney carcinoma using BID clone UMAB139, mouse monoclonal antibody at 1:100 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. UM500101 requires heat-induced epitope retrieval with Citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. The image shows strong nuclear, membranous, and cytoplasmic staining in the tumor cells.
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human melanoma using BID clone UMAB139, mouse monoclonal antibody at 1:100 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. UM500101 requires heat-induced epitope retrieval with Citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. The image shows strong nuclear, membranous, and cytoplasmic staining in the tumor cells.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human kidney carcinoma using BID clone UMAB139, mouse monoclonal antibody at 1:100 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. UM500101 requires heat-induced epitope retrieval with Citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. The image shows strong nuclear, membranous, and cytoplasmic staining in the tumor cells.
- Validation comment
- IHC
