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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP11299PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP11299PU-N, RRID:AB_1749185
- Product name
- anti BID (BH3 Domain)
- Antibody type
- Polyclonal
- Antigen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide encoding the BH3 domain of human Bid.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- LEFT: The anti-Bid BH3 domain Pab (Cat. #AP11299PU-N) is used in Western blot to detect Bid BH3 in HL-60 cell lysate. RIGHT: Western blot analysis of anti-hBid-BH3 Pab (Cat. #AP11299PU-N) in mouse lung tissue lysates (35ug/lane). hBid-BH3(arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- LEFT: Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. RIGHT: Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Bid BH3 Domain Antibody (Cat.#AP11299PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
