MAB860

antibody from R&D Systems

Targeting: BID

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB860

Provider

R&D Systems

Product name

Human/Mouse BID Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human and mouse BID in Western blots. Detects full-length mouse BID and the 15 kDa carboxyl-terminal fragment (tBID) generated by cleavage with Caspase-8. The antibody also detects human BID. However, in Western blots of recombinant human and mouse BID, the antibody is most sensitive for mouse BID.

Reactivity

Human, Mouse

Host

Rat

Conjugate

Unconjugated

Antigen sequence

P70444


Isotype

IgG

Antibody clone number

91508

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Impact of caspase-1/11, -3, -7, or IL-1β/IL-18 deficiency on rabies virus-induced macrophage cell death and onset of disease.

Kip E, Nazé F, Suin V, Vanden Berghe T, Francart A, Lamoral S, Vandenabeele P, Beyaert R, Van Gucht S, Kalai M
Cell death discovery 2017;3:17012

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Sensing cytosolic RpsL by macrophages induces lysosomal cell death and termination of bacterial infection.

Zhu W, Tao L, Quick ML, Joyce JA, Qu JM, Luo ZQ
PLoS pathogens 2015 Mar;11(3):e1004704

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The PI3K regulatory subunits p55α and p50α regulate cell death in vivo.

Pensa S, Neoh K, Resemann HK, Kreuzaler PA, Abell K, Clarke NJ, Reinheckel T, Kahn CR, Watson CJ
Cell death and differentiation 2014 Sep;21(9):1442-50

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BH3-only protein Bid is dispensable for seizure-induced neuronal death and the associated nuclear accumulation of apoptosis-inducing factor.

Engel T, Caballero-Caballero A, Schindler CK, Plesnila N, Strasser A, Prehn JH, Henshall DC
Journal of neurochemistry 2010 Oct;115(1):92-101

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Activation of the extrinsic caspase pathway in cultured cortical neurons requires p53-mediated down-regulation of the X-linked inhibitor of apoptosis protein to induce apoptosis.

Tun C, Guo W, Nguyen H, Yun B, Libby RT, Morrison RS, Garden GA
Journal of neurochemistry 2007 Aug;102(4):1206-19

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Proteolysis of HIP during apoptosis occurs within a region similar to the BID loop.

Caruso JA, Reiners JJ Jr
Apoptosis : an international journal on programmed cell death 2006 Nov;11(11):1877-85

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human and Mouse BID by Western Blot. Western blot shows lysates of Recombinant Mouse BID and Recombinant Human BID untreated (-) or treated (+) with Caspase-8. PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse BID Monoclonal Antibody (Catalog # MAB860) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands for BID were detected at approximately 12 kDa and 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human and Mouse BID by Western Blot. Western blot shows lysates of SVEC4-10 mouse vascular endothelial cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse BID Monoclonal Antibody (Catalog # MAB860) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for BID at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.