H00000637-M01

antibody from Abnova Corporation

Targeting: BID

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000637-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000637-M01, RRID:AB_425326

Product name

BID monoclonal antibody (M01), clone 3F3-1A3

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant BID.

Antigen sequence

MDCEVNNGSSLRDECITNLLVFGFLQSCSDNSFRR
ELDALGHELPVLAPQWEGYDELQTDGNRSSHSRLG
RIEADSESQEDIIRNIARHLAQVGDSMDRSIPPGL
VNGLALQLRNTSRSEEDRNRDLATALEQLLQAYPR
DMEKEKTMLVLALLLAKKVASHTPSLLRDVFHTTV
NFINQNLRTYVRSLARNGMD


Isotype

IgG

Antibody clone number

3F3-1A3

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of BID expression in transfected 293T cell line by BID monoclonal antibody (M01), clone 3F3-1A3.Lane 1: BID transfected lysate(22 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged BID is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between FADD and BID. HeLa cells were stained with anti-FADD rabbit purified polyclonal 1:1200 and anti-BID mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)