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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB0419 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0419, RRID:AB_982363
- Product name
- BID (phospho S65) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of BID.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The interaction between tBid and cardiolipin or monolysocardiolipin.
Specific cleavage of Mcl-1 by caspase-3 in tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in Jurkat leukemia T cells.
Bid mediates apoptotic synergy between tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and DNA damage.
Liu J, Durrant D, Yang HS, He Y, Whitby FG, Myszka DG, Lee RM
Biochemical and biophysical research communications 2005 May 13;330(3):865-70
Biochemical and biophysical research communications 2005 May 13;330(3):865-70
Specific cleavage of Mcl-1 by caspase-3 in tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in Jurkat leukemia T cells.
Weng C, Li Y, Xu D, Shi Y, Tang H
The Journal of biological chemistry 2005 Mar 18;280(11):10491-500
The Journal of biological chemistry 2005 Mar 18;280(11):10491-500
Bid mediates apoptotic synergy between tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and DNA damage.
Broaddus VC, Dansen TB, Abayasiriwardana KS, Wilson SM, Finch AJ, Swigart LB, Hunt AE, Evan GI
The Journal of biological chemistry 2005 Apr 1;280(13):12486-93
The Journal of biological chemistry 2005 Apr 1;280(13):12486-93
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- BID (phospho S65) polyclonal antibody (Cat # PAB0419). Western blot analysis of BID expression in human colon.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with BID (phospho S65) polyclonal antibody (Cat # PAB0419) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
