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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- PAB2442 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2442, RRID:AB_1571706
- Product name
- BID polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of BID.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Caspase 8-mediated cleavage of the pro-apoptotic BCL-2 family member BID in p53-dependent apoptosis.
Nonredundant role of Bax and Bak in Bid-mediated apoptosis.
Necrotic cell death in response to oxidant stress involves the activation of the apoptogenic caspase-8/bid pathway.
Fischer B, Coelho D, Dufour P, Bergerat JP, Denis JM, Gueulette J, Bischoff P
Biochemical and biophysical research communications 2003 Jun 27;306(2):516-22
Biochemical and biophysical research communications 2003 Jun 27;306(2):516-22
Nonredundant role of Bax and Bak in Bid-mediated apoptosis.
Cartron PF, Juin P, Oliver L, Martin S, Meflah K, Vallette FM
Molecular and cellular biology 2003 Jul;23(13):4701-12
Molecular and cellular biology 2003 Jul;23(13):4701-12
Necrotic cell death in response to oxidant stress involves the activation of the apoptogenic caspase-8/bid pathway.
Wang X, Ryter SW, Dai C, Tang ZL, Watkins SC, Yin XM, Song R, Choi AM
The Journal of biological chemistry 2003 Aug 1;278(31):29184-91
The Journal of biological chemistry 2003 Aug 1;278(31):29184-91
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The BID polyclonal antibody (Cat # PAB2442) is used in Western blot to detect BID in HL-60 cell lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of BID polyclonal antibody (Cat # PAB2442) in mouse lung tissue lysates (35 ug/lane). BID (arrow) was detected using the purified polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with BID polyclonal antibody (Cat # PAB2442) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the BID polyclonal antibody (Cat # PAB2442) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
