Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- A00730 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Bid Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for Bid detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC-P. Reactive species: Mouse. Bid information: Molecular Weight: 21952 MW; Subcellular Localization: Cytoplasm . Mitochondrion membrane . When uncleaved, it is predominantly cytoplasmic. p15 BID translocates to mitochondria as an integral membrane protein. p13 and p22 BID are associated with the mitochondrial membrane.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Vial size
- 100ug/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Aconitine induces cell apoptosis via mitochondria and death receptor signaling pathways in hippocampus cell line.
Circular RNA Rbms1 inhibited the development of myocardial ischemia reperfusion injury by regulating miR-92a/BCL2L11 signaling pathway.
Wang H, Liu Y, Guo Z, Wu K, Zhang Y, Tian Y, Zhao B, Lu H
Research in veterinary science 2022 Mar;143:124-133
Research in veterinary science 2022 Mar;143:124-133
Circular RNA Rbms1 inhibited the development of myocardial ischemia reperfusion injury by regulating miR-92a/BCL2L11 signaling pathway.
Jin L, Zhang Y, Jiang Y, Tan M, Liu C
Bioengineered 2022 Feb;13(2):3082-3092
Bioengineered 2022 Feb;13(2):3082-3092
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of Bid using anti-Bid antibody (A00730). Bid was detected in paraffin-embedded section of mouse spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Bid Antibody (A00730) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image