ABIN358089

antibody from antibodies-online

Targeting: BID

Western blot (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

ABIN358089

Provider

antibodies-online

Product name

anti-BH3 Interacting Domain Death Agonist (BID) (pSer65) antibody

Antibody type

Polyclonal

Description

Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Reactivity

Human

Host

Rabbit

Epitope

pSer65

Vial size

0.4 mL

Storage

Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.

Handling

Avoid repeated freezing and thawing.

References

Submitted references

The interaction between tBid and cardiolipin or monolysocardiolipin.

Liu J, Durrant D, Yang HS, He Y, Whitby FG, Myszka DG, Lee RM
Biochemical and biophysical research communications 2005 May 13;330(3):865-70

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Specific cleavage of Mcl-1 by caspase-3 in tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in Jurkat leukemia T cells.

Weng C, Li Y, Xu D, Shi Y, Tang H
The Journal of biological chemistry 2005 Mar 18;280(11):10491-500

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Bid mediates apoptotic synergy between tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and DNA damage.

Broaddus VC, Dansen TB, Abayasiriwardana KS, Wilson SM, Finch AJ, Swigart LB, Hunt AE, Evan GI
The Journal of biological chemistry 2005 Apr 1;280(13):12486-93

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Conformation of membrane-associated proapoptotic tBid.

Gong XM, Choi J, Franzin CM, Zhai D, Reed JC, Marassi FM
The Journal of biological chemistry 2004 Jul 9;279(28):28954-60

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Membrane-insertion fragments of Bcl-xL, Bax, and Bid.

García-Sáez AJ, Mingarro I, Pérez-Payá E, Salgado J
Biochemistry 2004 Aug 31;43(34):10930-43