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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 650502 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#650502, RRID:AB_10897951
- Product name
- Purified anti-BID
- Antibody type
- Monoclonal
- Antigen
- Bacteria expressed His tagged full length recombinant protein
- Description
- BID, a BH3 interacting domain death agonist is a member of the Bcl-2/Bcl-xL family. BID can form a heterodimer with either agonist BAX or antagonist Bcl-2. It is a mediator of mitochondrial damage induced by Caspase-8 (CASP8). After the cleavage by CASP8, the COOH-terminal part translocates to mitochondria and induces cytochrome c release, which in turn activates downstream caspases. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Upon receipt, store undiluted at 4°C.
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Jurkat cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-BID antibody (clone 1H11). Proteins were visualized using a goat anti-rat IgG secondary conjugated to HRP and chemiluminescence detection.
- Conjugate
- Unconjugated
