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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PAB0278 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0278, RRID:AB_1019087
- Product name
- Traf5 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of Traf5.
- Antigen sequence
MAHSEEQAAVPCAFIRQNSG- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references TNFR-associated factor family protein expression in normal tissues and lymphoid malignancies.
Zapata JM, Krajewska M, Krajewski S, Kitada S, Welsh K, Monks A, McCloskey N, Gordon J, Kipps TJ, Gascoyne RD, Shabaik A, Reed JC
Journal of immunology (Baltimore, Md. : 1950) 2000 Nov 1;165(9):5084-96
Journal of immunology (Baltimore, Md. : 1950) 2000 Nov 1;165(9):5084-96
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of Traf5 in normal human tissues. Using Traf5 polyclonal antibody (Cat # PAB0278) at 1 : 2000. Traf5 is observed at ~64 KDa. Additional bands of lower molecular weight were seen in some cases, and may represent Traf5 degradation fragments.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded mouse tissue stained for Traf5 expression. Using Traf5 polyclonal antibody (Cat # PAB0278) at 1 : 2000. Hematoxylin-eosin counterstain. A, thymus. B, bone marrow.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded mouse spleen stained for Traf5 expression. Using Traf5 polyclonal antibody (Cat # PAB0278) at 1 : 2000. Hematoxylin-eosin counterstain. A1 is a higher magnification of A.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
