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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [17]
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- Western blot [3]
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- Product number
- AF860 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse BID Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human and mouse BID, a fragment generated in cells treated with anti-Fas and the carboxyl-terminal portion of recombinant BID generated by cleavage with Caspase 8 in Western blots.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
AAC71064- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Single-cell analysis of pyroptosis dynamics reveals conserved GSDMD-mediated subcellular events that precede plasma membrane rupture.
Mitochondrial fusion and Bid-mediated mitochondrial apoptosis are perturbed by alcohol with distinct dependence on its metabolism.
Highly tumorigenic hepatocellular carcinoma cell line with cancer stem cell-like properties.
Executioner caspases and CAD are essential for mutagenesis induced by TRAIL or vincristine.
Germline quality control: eEF2K stands guard to eliminate defective oocytes.
Platelet-derived growth factor primes cancer-associated fibroblasts for apoptosis.
GLI3-dependent repression of DR4 mediates hedgehog antagonism of TRAIL-induced apoptosis.
Down-regulation of FoxO-dependent c-FLIP expression mediates TRAIL-induced apoptosis in activated hepatic stellate cells.
Cardioprotection against myocardial infarction with PTD-BIR3/RING, a XIAP mimicking protein.
Sensitization of imatinib-resistant CML cells to TRAIL-induced apoptosis is mediated through down-regulation of Bcr-Abl as well as c-FLIP.
Trichostatin A sensitizes human ovarian cancer cells to TRAIL-induced apoptosis by down-regulation of c-FLIPL via inhibition of EGFR pathway.
Yersinia YopP-induced apoptotic cell death in murine dendritic cells is partially independent from action of caspases and exhibits necrosis-like features.
Translocation of full-length Bid to mitochondria during anoikis.
Early events in the anoikis program occur in the absence of caspase activation.
Post-translational modification of Bid has differential effects on its susceptibility to cleavage by caspase 8 or caspase 3.
Methamphetamine causes differential regulation of pro-death and anti-death Bcl-2 genes in the mouse neocortex.
Methamphetamine causes differential regulation of pro-death and anti-death Bcl-2 genes in the mouse neocortex.
de Vasconcelos NM, Van Opdenbosch N, Van Gorp H, Parthoens E, Lamkanfi M
Cell death and differentiation 2019 Jan;26(1):146-161
Cell death and differentiation 2019 Jan;26(1):146-161
Mitochondrial fusion and Bid-mediated mitochondrial apoptosis are perturbed by alcohol with distinct dependence on its metabolism.
Naghdi S, Slovinsky WS, Madesh M, Rubin E, Hajnóczky G
Cell death & disease 2018 Oct 9;9(10):1028
Cell death & disease 2018 Oct 9;9(10):1028
Highly tumorigenic hepatocellular carcinoma cell line with cancer stem cell-like properties.
Lacoste B, Raymond VA, Cassim S, Lapierre P, Bilodeau M
PloS one 2017;12(2):e0171215
PloS one 2017;12(2):e0171215
Executioner caspases and CAD are essential for mutagenesis induced by TRAIL or vincristine.
Miles MA, Hawkins CJ
Cell death & disease 2017 Oct 5;8(10):e3062
Cell death & disease 2017 Oct 5;8(10):e3062
Germline quality control: eEF2K stands guard to eliminate defective oocytes.
Chu HP, Liao Y, Novak JS, Hu Z, Merkin JJ, Shymkiv Y, Braeckman BP, Dorovkov MV, Nguyen A, Clifford PM, Nagele RG, Harrison DE, Ellis RE, Ryazanov AG
Developmental cell 2014 Mar 10;28(5):561-572
Developmental cell 2014 Mar 10;28(5):561-572
Platelet-derived growth factor primes cancer-associated fibroblasts for apoptosis.
Ilyas SI, Mertens JC, Bronk SF, Hirsova P, Dai H, Roberts LR, Kaufmann SH, Gores GJ
The Journal of biological chemistry 2014 Aug 15;289(33):22835-22849
The Journal of biological chemistry 2014 Aug 15;289(33):22835-22849
GLI3-dependent repression of DR4 mediates hedgehog antagonism of TRAIL-induced apoptosis.
Kurita S, Mott JL, Almada LL, Bronk SF, Werneburg NW, Sun SY, Roberts LR, Fernandez-Zapico ME, Gores GJ
Oncogene 2010 Aug 26;29(34):4848-58
Oncogene 2010 Aug 26;29(34):4848-58
Down-regulation of FoxO-dependent c-FLIP expression mediates TRAIL-induced apoptosis in activated hepatic stellate cells.
Park SJ, Sohn HY, Yoon J, Park SI
Cellular signalling 2009 Oct;21(10):1495-503
Cellular signalling 2009 Oct;21(10):1495-503
Cardioprotection against myocardial infarction with PTD-BIR3/RING, a XIAP mimicking protein.
Souktani R, Pons S, Guegan C, Bouhidel O, Bruneval P, Zini R, Mandet C, Onteniente B, Berdeaux A, Ghaleh B
Journal of molecular and cellular cardiology 2009 May;46(5):713-8
Journal of molecular and cellular cardiology 2009 May;46(5):713-8
Sensitization of imatinib-resistant CML cells to TRAIL-induced apoptosis is mediated through down-regulation of Bcr-Abl as well as c-FLIP.
Park SJ, Kim MJ, Kim HB, Kang CD, Kim SH
The Biochemical journal 2009 Apr 28;420(1):73-81
The Biochemical journal 2009 Apr 28;420(1):73-81
Trichostatin A sensitizes human ovarian cancer cells to TRAIL-induced apoptosis by down-regulation of c-FLIPL via inhibition of EGFR pathway.
Park SJ, Kim MJ, Kim HB, Sohn HY, Bae JH, Kang CD, Kim SH
Biochemical pharmacology 2009 Apr 15;77(8):1328-36
Biochemical pharmacology 2009 Apr 15;77(8):1328-36
Yersinia YopP-induced apoptotic cell death in murine dendritic cells is partially independent from action of caspases and exhibits necrosis-like features.
Gröbner S, Autenrieth SE, Soldanova I, Gunst DS, Schaller M, Bohn E, Müller S, Leverkus M, Wesselborg S, Autenrieth IB, Borgmann S
Apoptosis : an international journal on programmed cell death 2006 Nov;11(11):1959-68
Apoptosis : an international journal on programmed cell death 2006 Nov;11(11):1959-68
Translocation of full-length Bid to mitochondria during anoikis.
Valentijn AJ, Gilmore AP
The Journal of biological chemistry 2004 Jul 30;279(31):32848-57
The Journal of biological chemistry 2004 Jul 30;279(31):32848-57
Early events in the anoikis program occur in the absence of caspase activation.
Wang P, Valentijn AJ, Gilmore AP, Streuli CH
The Journal of biological chemistry 2003 May 30;278(22):19917-25
The Journal of biological chemistry 2003 May 30;278(22):19917-25
Post-translational modification of Bid has differential effects on its susceptibility to cleavage by caspase 8 or caspase 3.
Degli Esposti M, Ferry G, Masdehors P, Boutin JA, Hickman JA, Dive C
The Journal of biological chemistry 2003 May 2;278(18):15749-57
The Journal of biological chemistry 2003 May 2;278(18):15749-57
Methamphetamine causes differential regulation of pro-death and anti-death Bcl-2 genes in the mouse neocortex.
Jayanthi S, Deng X, Bordelon M, McCoy MT, Cadet JL
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2001 Aug;15(10):1745-52
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2001 Aug;15(10):1745-52
Methamphetamine causes differential regulation of pro-death and anti-death Bcl-2 genes in the mouse neocortex.
Jayanthi S, Deng X, Bordelon M, McCoy MT, Cadet JL
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2001 Aug;15(10):1745-52
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2001 Aug;15(10):1745-52
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human BID by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for BID at approximately 29 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse BID Antigen Affinity-purified Polyclonal Antibody (Catalog # AF860) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human BID by Western Blot. Western blot shows Jurkat human acute T cell leukemia cell line treated with apoptosis inducer anti-Fas for the indicated times. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse BID Antigen Affinity-purified Polyclonal Antibody (Catalog # AF860) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BID at approximately 20 kDa (as indicated). For additional reference short (5 seconds, left panel) and long (60 seconds, right panel) exposures are shown. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Mouse BID by Western Blot. Western blot shows lysates of SVEC4-10 mouse vascular endothelial cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse BID Antigen Affinity-purified Polyclonal Antibody (Catalog # AF860) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for BID at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
