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Western blot
Immunoprecipitation
Other assayAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Other assay [1]
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- Product number
- PA5-48040 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NETO2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant human NETO1 is observed. Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunoprecipitation of NETO2 was performed using mouse brain tissue. Antigen-antibody complexes were formed by incubating 500 µg lysates of mouse brain tissue with NETO2 polyclonal antibody (Product # PA5-48040) for 8 hours at 2‚8 °C using a dilution of 1‚2 µg/mL. NETO2-antibody complexes were absorbed using Protein G. Immunoprecipitated NETO2 was detected by Western blot using 1 µg/mL Goat Anti-Human/Mouse NETO2 Antigen Affinity-purified Polyclonal Antibody.
