Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AF6498 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Integrin alpha 11 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Integrin alpha 11 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human (rh) Integrin alpha 11 is observed, and less than 2% cross-reactivity with recombinant mouse (rm) Integrin alpha 2 and rmIntegrin alpha 10 is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P61622
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Highlights of glycosylation and adhesion related genes involved in myogenesis.
Grassot V, Da Silva A, Saliba J, Maftah A, Dupuy F, Petit JM
BMC genomics 2014 Jul 22;15:621
BMC genomics 2014 Jul 22;15:621
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse Integrin alpha 11 by Western Blot. Western blot shows lysates of mouse embryo tissue. PVDF Membrane was probed with 1 µg/mL of Mouse Integrin alpha 11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6498) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Integrin alpha 11 at approximately 230 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Integrin alpha 11 in Mouse Embryonic Fibroblasts (iMEF). Integrin alpha 11 was detected in immersion fixed irradiated mouse embryonic fibroblasts (iMEF) using Mouse Integrin alpha 11 Affinity-purified Polyclonal Antibody (Catalog # AF6498) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.