PA5-28951

antibody from Invitrogen Antibodies

Targeting: SAR1B SARA2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28951

Provider

Invitrogen Antibodies

Product name

SAR1B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: HeLa, mouse brain, rat brain.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using SAR1B Polyclonal Antibody (Product # PA5-28951). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. SAR1B Polyclonal Antibody (Product # PA5-28951) diluted at 1:3,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SAR1B Polyclonal Antibody detects SAR1B protein by western blot analysis. A. 50 µg rat brain lysate/extract.12% SDS-PAGE. SAR1B Polyclonal Antibody (Product # PA5-28951) dilution: 1:3,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using SAR1B Polyclonal Antibody (Product # PA5-28951). Sample (30 µg of whole cell lysate). A: HeLa. 12% SDS PAGE. SAR1B Polyclonal Antibody (Product # PA5-28951) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SAR1B in paraformaldehyde-fixed HeLa cells using a SAR1B polyclonal antibody (Product # PA5-28951) at a 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of SAR1B was performed in paraffin-embedded mouse ovary tissue using SAR1B Polyclonal Antibody (Product # PA5-28951) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using GTBPB (Product # PA5-28951) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.