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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-28951 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SAR1B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: HeLa, mouse brain, rat brain. Predicted reactivity: Mouse (98%), Rat (98%), Zebrafish (91%), Xenopus laevis (85%), Pig (98%), Chicken (95%), Rhesus Monkey (99%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SAR1B Polyclonal Antibody (Product # PA5-28951). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. SAR1B Polyclonal Antibody (Product # PA5-28951) diluted at 1:3,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SAR1B Polyclonal Antibody detects SAR1B protein by western blot analysis. A. 50 µg rat brain lysate/extract.12% SDS-PAGE. SAR1B Polyclonal Antibody (Product # PA5-28951) dilution: 1:3,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SAR1B Polyclonal Antibody (Product # PA5-28951). Sample (30 µg of whole cell lysate). A: HeLa. 12% SDS PAGE. SAR1B Polyclonal Antibody (Product # PA5-28951) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SAR1B in paraformaldehyde-fixed HeLa cells using a SAR1B polyclonal antibody (Product # PA5-28951) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of SAR1B was performed in paraffin-embedded mouse ovary tissue using SAR1B Polyclonal Antibody (Product # PA5-28951) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using GTBPB (Product # PA5-28951) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
