A301-677A

antibody from Invitrogen Antibodies

Targeting: CCNL2 ania-6b, CCNM, CCNS, HLA-ISO, PCEE, SB138

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A301-677A

Provider

Invitrogen Antibodies

Product name

Cyclin L2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.20 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Cyclin L2 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, K-562, HeLa, U2OS, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-Cyclin L2 antibody (Product # A301-677A; lot A301-677A-2) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Cyclin L2 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Cyclin L2 antibody A301-677A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. Cyclin L2 was also immunoprecipitated by rabbit anti-Cyclin L2 antibody A301-676A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Cyclin L2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hela cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-Cyclin L2 antibody (Product # A301-677A; lot A301-677A-2) used for IP at 6 µg per reaction. Cyclin L2 was also immunoprecipitated by a previous lot of this antibody (lot A301-677A-1) and rabbit anti-Cyclin L2 antibody BL6436. For blotting immunoprecipitated Cyclin L2, Product # A301-677A was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.