Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
Reference
Comment
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- Product number
- GTX85686 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX85686, RRID:AB_10732293
- Product name
- CCNL2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Rat, Chicken/Avian
- Host
- Rabbit
- Storage
- +4¢XC or -20¢XC, Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot of Affinity Purified anti-Cyclin L2a antibody shows detection of a band ~38 kDa corresponding to a GST-truncated human Cyclin L2a fusion protein (arrowhead). Approximately 5 ul of an induced E.coli cell lysate expressing recombinant Cyclin L2 was separated by 4-20% SDS-PAGE followed by transfer to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1000 overnight at 4C followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Human Small Intestine (formalin-fixed, paraffin-embedded) stained with CCNL2 antibody (GTX85686) at 5 μg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.