HPA000412

antibody from Atlas Antibodies

Targeting: ARHGAP12 FLJ10971, FLJ20737, FLJ21785

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA000412

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA000412, RRID:AB_10964051

Product name

Anti-ARHGAP12

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human ARHGAP12, Gene description: Rho GTPase activating protein 12, Alternative Gene Names: FLJ10971, FLJ20737, FLJ21785, Validated applications: WB, IHC, Uniprot ID: Q8IWW6, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.4 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

RPEL-family rhoGAPs link Rac/Cdc42 GTP loading to G-actin availability

Diring J, Mouilleron S, McDonald N, Treisman R
Nature Cell Biology 2019;21(7):845-855

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Phosphoinositide 3-kinase enables phagocytosis of large particles by terminating actin assembly through Rac/Cdc42 GTPase-activating proteins

Schlam D, Bagshaw R, Freeman S, Collins R, Pawson T, Fairn G, Grinstein S
Nature Communications 2015;6(1)

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human stomach and skeletal muscle tissues using HPA000412 antibody. Corresponding ARHGAP12 RNA-seq data are presented for the same tissues.

Sample type

Human

Protocol

Protocol

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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