HPA000412

antibody from Atlas Antibodies

Targeting: ARHGAP12 FLJ10971, FLJ20737, FLJ21785

Western blot (Data presented on provider website)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA000412

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA000412, RRID:AB_10964051

Product name

Anti-ARHGAP12

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

RGTQERTWKPPRWTRDASISKGDFQNPGDQELLSS
EENYYSTSYSQSDSQCGSPPRGWSEELDERGHTLY
TSDYTNEKWLKHVDDQGRQYYYSADGSRSEWELPK
YNASSQQQREIIKSRSLDRRLQEPIVLTKWRHSTI
VLDTNDKESPTASKPCFPENESSPSSP


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

Submitted references

Phosphoinositide 3-kinase enables phagocytosis of large particles by terminating actin assembly through Rac/Cdc42 GTPase-activating proteins.

Schlam D, Bagshaw RD, Freeman SA, Collins RF, Pawson T, Fairn GD, Grinstein S
Nature communications 2015 Oct 14;6:8623

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human stomach and skeletal muscle tissues using Anti-ARHGAP12 antibody. Corresponding ARHGAP12 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human stomach shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows low expression as expected.

Sample type

HUMAN