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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AMR-004-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHRM4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.85 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain membrane (lanes 1 and 2), mouse brain membrane (lane 3 and 5) and SH-SY5Y cell lysate (lanes 4 and 6): - 1,3,4. Anti-CHRM4 Antibody (#AMR-004), (1:200).2,5,6. Anti-CHRM4 Antibody , preincubated with CHRM4 Blocking Peptide (#BLP-MR004).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of Muscarinic acetylcholine receptorM4in C6 transfected cells - Immunocytochemical staining of paraformaldehyde-fixed and permeabilized C6 cells. A. Transfected cells were stained using Anti-CHRM4 Antibody (#AMR-004), (1:100) followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody (red). B. Non-transfected cells were stained using Anti-CHRM4 Antibody (1:100), followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody (red). DAPI is used as the counterstain (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression ofMuscarinic acetylcholine receptorM4in mouse islands of Calleja - Immunohistochemical staining of mouse islands of Calleja (ventral striatum) using Anti-CHRM4 Antibody (#AMR-004). A. M4 mAChR staining (red) appears in soma of neuronal profiles (vertical arrows) and a diffuse halo (horizontal arrows) in the vicinity of the islands of Calleja (IC). B. Nuclear staining using DAPI as the counterstain. C. Merged image of A and B.
