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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-60824 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GSPT2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: TQPPTLPAGS GSNDETCTGA GYPQGKRMGR GAPVEPSREE PLVSLEGSNS AVT Highest antigen sequence identity to the following orthologs: Mouse - 63%, Rat - 64%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Effect of small molecule eRF3 degraders on premature termination codon readthrough.
Baradaran-Heravi A, Balgi AD, Hosseini-Farahabadi S, Choi K, Has C, Roberge M
Nucleic acids research 2021 Apr 19;49(7):3692-3708
Nucleic acids research 2021 Apr 19;49(7):3692-3708
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GSPT2 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a GSPT2 Polyclonal Antibody (Product # PA5-60824).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of GSPT2 in human cell line PC-3 shows positivity in cytoplasm. Samples were probed using a GSPT2 Polyclonal Antibody (Product # PA5-60824).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GSPT2 in human rectal tissue shows moderate cytoplasmic positivity in glandular cells. Samples were probed using a GSPT2 Polyclonal Antibody (Product # PA5-60824).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 8. Effect of CC-885 and CC-90009 on COL17A1 PTC readthrough. ( A, B ) HaCaT keratinocytes derived from an unaffected individual were exposed to various concentrations of CC-885 ( A ) or CC-90009 ( B ) with or without 100 mug/ml gentamicin sulfate for 48 h and cell viability was measured using the MTT assay in triplicate samples (+-SD). Concentrations of CC-885 and CC-90009 are in nanomolar. ( C, D ) JEB01 keratinocytes derived from a JEB patient with a COL17A1 nonsense mutation (R688X/R688X) were exposed to the indicated concentrations of CC-885 ( C ) or CC-90009 ( D ) for 72 h and eRF3a, eRF3b and eRF1 levels were determined using automated capillary electrophoresis western analysis. GAPDH was used as loading control. ( E, F ) JEB01 keratinocytes were exposed to the indicated concentrations of CC-885 ( E ) or CC-90009 ( F ) in the presence or absence of 100 or 300 mug/ml gentamicin sulfate for 72 h and Collagen XVII levels were measured by SDS-PAGE followed by traditional western blotting. beta -actin was used as loading control.
