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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Flow cytometry [2]
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Validation data
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- Product number
- TA503066 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA503066, RRID:AB_11140124
- Product name
- GSPT2 mouse monoclonal antibody, clone OTI2G9 (formerly 2G9)
- Antibody type
- Monoclonal
- Description
- GSPT2 mouse monoclonal antibody, clone OTI2G9 (formerly 2G9)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- GSPT2
- Isotype
- IgG
- Antibody clone number
- OTI2G9
- Vial size
- 100 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GSPT2 (RC206259, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GSPT2.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-GSPT2 monoclonal antibody.(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-GSPT2 mouse monoclonal antibody (TA503066) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GSPT2(RC206259).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-GSPT2 mouse monoclonal antibody (TA503066).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC206259 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GSPT2 antibody(TA503066), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-GSPT2 antibody(TA503066),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
