12989-1-AP

antibody from Invitrogen Antibodies

Targeting: GSPT2 eRF3b, FLJ10441

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

12989-1-AP

Provider

Invitrogen Antibodies

Product name

GSPT2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MDSGSSSSD SAPDCWDQVD MESTGSAPSG DGVSSAVAEA QREPLSSAFS RKLNVNAKPF VPNVHAAEFV PSFLRGPTQP PTLPAGSGSN DETCTGAGYP QGKRMGRGAP VEPSREEPLV SLEGSNSAVT MELSEPVVEN GEVEMALEES WEHSKEVSEA EPGGGSSGDS GPPEESGQEM MEEKEEIRKS KSVIVPSGAP K (1-200 aa encoded by BC036077)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.25 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF7 cells were subjected to SDS PAGE followed by western blot with 12989-1-AP (GSPT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12989-1-AP (GSPT2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed MCF-7 cells using 12989-1-AP (GSPT2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed MCF-7 cells using 12989-1-AP (GSPT2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using 12989-1-AP (GSPT2 antibody) at dilution of 1:100 (under 10x lens).