13762-1-AP

antibody from Proteintech Group

Targeting: AMBRA1 DCAF3, FLJ20294, KIAA1736, WDR94

Provider product page for 13762-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [26]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [1]

Submit

Product number: 13762-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#13762-1-AP, RRID:AB_10642007
Product name: AMBRA1 antibody
Antibody type: Polyclonal
Description: KD/KO validated AMBRA1 antibody (Cat. #13762-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

AMBRA1 protein structure - 13762-1-AP shown in red.

Submitted references AMBRA1 enhances Sonic Hedgehog signaling during cerebellar development and in medulloblastoma.

Basili I, Bufalieri F, Conenna M, Navacci S, Li YR, Torrejon J, Bernardi F, Lospinoso Severini L, Marsaud V, Cancila G, Bourmeau G, Yu H, Lo Re V, Talbot J, Adabbo G, Di Pinto A, Agnoli F, Giovannini D, Leonardi S, Bordone R, Petroni M, Souphron J, Blauwblomme T, Beccaria K, Dufour C, D'Angelo L, De Smaele E, Canettieri G, Giannini G, Fimia GM, Maroder M, Guardavaccaro D, Pazzaglia S, Tsai JW, Infante P, Ayrault O, Di Marcotullio L
Developmental cell 2026 May 13;61(5):1111-1126.e9

Interaction of CSN4 to DDB1 regulates its stability and function in DNA damage signaling.

Choi SH, Cho K, Kim ES, Yoo HY
Cellular and molecular life sciences : CMLS 2026 Feb 13;83(1):111

Triflupromazine and tranylcypromine alleviate primary cisplatin resistance in lung adenocarcinoma by promoting LDHA-mediated AMBRA1 ubiquitination.

Wang Q, Zhang Y, Zhang Y, Zhai Y, Zhou X, Qin Y, Ding M, Tian Y, Zhang Z, Zhang P, Xu F
Biochemical pharmacology 2025 Sep;239:117073

Redox-Induced Stabilization of AMBRA1 by USP7 Promotes Intestinal Oxidative Stress and Colitis Through Antagonizing DUB3-Mediated NRF2 Deubiquitination.

Xu W, Hua Z, Wang Y, Tang W, Ge W, Chen Y, Wang Z, Gu Y, Liu CY, Du P
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Mar;12(12):e2411320

METTL3 regulates Ambra1 expression in an m6A-YTHDF2-dependent manner to promote mantle cell lymphoma progression.

Li S, Jiang Z, Wei W, Deng S, Duan T
Journal of translational medicine 2025 Jul 1;23(1):703

POU4F1 enhances lung cancer gemcitabine resistance by regulating METTL3-dependent TWF1 mRNA N6 adenosine methylation.

Tang J, Liu Z, Xie G, Wang C, Jiang Y
3 Biotech 2025 Jan;15(1):7

Stabilization of GTSE1 by cyclin D1-CDK4/6-mediated phosphorylation promotes cell proliferation: relevance in cancer prognosis.

García-Vázquez N, González-Robles TJ, Lane E, Spasskaya D, Zhang Q, Kerzhnerman M, Jeong Y, Collu M, Simoneschi D, Ruggles KV, Rona G, Kaisari S, Pagano M
bioRxiv : the preprint server for biology 2025 Jan 23;

F-box protein FBXO32 ubiquitinates and stabilizes D-type cyclins to drive cancer progression.

Li F, Yu H, Zhang Y, Ma Y, Chen X, Zhang J, Sun L, Guo R, Wu Y, Zheng P, Wang X, Bie P, He F, Zhang L, Xie C, Xiong H
Nature communications 2025 Apr 30;16(1):4060

Stabilization of GTSE1 by cyclin D1-CDK4/6-mediated phosphorylation promotes cell proliferation with implications for cancer prognosis.

García-Vázquez N, González-Robles TJ, Lane E, Spasskaya D, Zhang Q, Kerzhnerman MA, Jeong Y, Collu M, Simoneschi D, Ruggles KV, Róna G, Kaisari S, Pagano M
eLife 2025 Apr 24;13

CDK4/6 Inhibitors Impede Chemoresistance and Inhibit Tumor Growth of Small Cell Lung Cancer.

Wen Y, Sun X, Zeng L, Liang S, Li D, Chen X, Zeng F, Zhang C, Wang Q, Zhong Q, Deng L, Guo L
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Oct;11(38):e2400666

Rab40 GTPases regulate AMBRA1-mediated transcription and cell migration.

Sampath R, Vaeth K, Mikalayeva V, Skeberdis VA, Prekeris R, Han KJ
bioRxiv : the preprint server for biology 2024 Nov 7;

AMBRA1 promotes intestinal inflammation by antagonizing PP4R1/PP4c mediated IKK dephosphorylation in an autophagy-independent manner.

Xu W, Hua Z, Wang Y, Tang W, Ou W, Liu F, Yang Y, Ding W, Wang Z, Cui L, Ge W, Gu Y, Wang X, Chen Y, Liu CY, Du P
Cell death and differentiation 2024 May;31(5):618-634

Ambra1 in exosomes secreted by HK-2 cells damaged by supersaturated oxalate induce mitophagy and autophagy-ferroptosis in normal HK-2 cells to participate in the occurrence of kidney stones.

Su X, Song C, He Z, Song Q, Meng L, Dong C, Zhou J, Ke H, Xiong Y, Liu J, Liao W, Yang S
Biochimica et biophysica acta. Molecular cell research 2024 Jan;1871(1):119604

Disrupting the interaction between AMBRA1 and DLC1 prevents apoptosis while enhancing autophagy and mitophagy.

Hawkins K, Watt M, Gillotin S, Hanspal M, Helley M, Richardson J, Corbett N, Brownlees J
Biology open 2024 Dec 15;13(12)

MiR-3653 blocks autophagy to inhibit epithelial-mesenchymal transition in breast cancer cells by targeting the autophagy-regulatory genes ATG12 and AMBRA1.

Song H, Zhao Z, Ma L, Zhang B, Song Y
Chinese medical journal 2023 Sep 5;136(17):2086-2100

AMBRA1 attenuates the proliferation of uveal melanoma cells.

Zhao B, Yang Y, Cun B, Chen P
Open medicine (Warsaw, Poland) 2022;17(1):1-14

Oxidative Stress-induced Autophagy Compromises Stem Cell Viability.

Prakash R, Fauzia E, Siddiqui AJ, Yadav SK, Kumari N, Shams MT, Naeem A, Praharaj PP, Khan MA, Bhutia SK, Janowski M, Boltze J, Raza SS
Stem cells (Dayton, Ohio) 2022 May 27;40(5):468-478

Shikonin induces apoptosis and autophagy via downregulation of pyrroline-5-carboxylate reductase1 in hepatocellular carcinoma cells.

Zhang J, Shang L, Jiang W, Wu W
Bioengineered 2022 Mar;13(3):7904-7918

Intron retention is a stress response in sensor genes and is restored by Japanese herbal medicines: A basis for future clinical applications.

Vu TD, Ito N, Oshima K, Maruko A, Nishi A, Mizoguchi K, Odaguchi H, Kobayashi Y, Okada N
Gene 2022 Jul 1;830:146496

Neuron-astrocyte transmitophagy is altered in Alzheimer's disease.

Lampinen R, Belaya I, Saveleva L, Liddell JR, Rait D, Huuskonen MT, Giniatullina R, Sorvari A, Soppela L, Mikhailov N, Boccuni I, Giniatullin R, Cruz-Haces M, Konovalova J, Koskuvi M, Domanskyi A, Hämäläinen RH, Goldsteins G, Koistinaho J, Malm T, Chew S, Rilla K, White AR, Marsh-Armstrong N, Kanninen KM
Neurobiology of disease 2022 Aug;170:105753

Zhang J, Ding R, Wu T, Jia J, Cheng X
Frontiers in cell and developmental biology 2021;9:705291

N-linoleyltyrosine exerts neuroprotective effects in APP/PS1 transgenic mice via cannabinoid receptor-mediated autophagy.

Long CM, Zheng QX, Zhou Y, Liu YT, Gong LP, Zeng YC, Liu S
Journal of pharmacological sciences 2021 Dec;147(4):315-324

Selenoprotein S attenuates high glucose and/or ox-LDL-induced endothelium injury by regulating Akt/mTOR signaling and autophagy.

Wang Z, Li Y, Yao J, Yu S, Yu H, Men L, Du J
The international journal of biochemistry & cell biology 2021 Dec;141:106111

CRL4(AMBRA1) is a master regulator of D-type cyclins.

Simoneschi D, Rona G, Zhou N, Jeong YT, Jiang S, Milletti G, Arbini AA, O'Sullivan A, Wang AA, Nithikasem S, Keegan S, Siu Y, Cianfanelli V, Maiani E, Nazio F, Cecconi F, Boccalatte F, Fenyö D, Jones DR, Busino L, Pagano M
Nature 2021 Apr;592(7856):789-793

miR-103a-3p regulates mitophagy in Parkinson's disease through Parkin/Ambra1 signaling.

Zhou J, Zhao Y, Li Z, Zhu M, Wang Z, Li Y, Xu T, Feng D, Zhang S, Tang F, Yao J
Pharmacological research 2020 Oct;160:105197

AMBRA1, a novel α-synuclein-binding protein, is implicated in the pathogenesis of multiple system atrophy.

Miki Y, Tanji K, Mori F, Tatara Y, Utsumi J, Sasaki H, Kakita A, Takahashi H, Fimia GM, Wakabayashi K
Brain pathology (Zurich, Switzerland) 2018 Jan;28(1):28-42

No comments: Submit comment

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: mouse brain tissue were subjected to SDS PAGE followed by western blot with 13762-1-AP(AMBRA1 antibody) at dilution of 1:300
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The AMBRA1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human AMBRA1. This antibody recognizes human,mouse,rat antigen. The AMBRA1 antibody has been validated for the following applications: ELISA, IHC, WB analysis.
Appendix: 13762-1-AP.pdf

: Show more