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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- TA328000 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal anti-PERK Phospho (Ser713) Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal anti-PERK Phospho (Ser713) Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- EIF2AK3
- Isotype
- IgG
- Antibody clone number
- Poly6494
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
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Supportive validation
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- OriGene (provider)
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- Experimental details
- 293E cells were transfected with PERK-FLAG construct. Cell lysates were then immunoprecipitated with L5 antibody. Proteins were then treated with calf intestinal alkaline phosphatase (CIP). The proteins without CIP treatment were used as the control. Samples were separated by electrophoresis, transferred to nitrocellulose and probed with anti-PERK phospho (Ser713) antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminenescent system.
- Validation comment
- WB
