Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [3]
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- Product number
- PA5-40294 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-PERK (Thr982) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with mouse and rat based on sequence homology.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Dihydroartemisinin triggers ferroptosis in primary liver cancer cells by promoting and unfolded protein response‑induced upregulation of CHAC1 expression.
Targeted Inhibition of P4HB Promotes Cell Sensitivity to Gemcitabine in Urothelial Carcinoma of the Bladder.
Towards Age-Related Anti-Inflammatory Therapy: Klotho Suppresses Activation of ER and Golgi Stress Response in Senescent Monocytes.
Knockdown of cytokeratin 8 overcomes chemoresistance of chordoma cells by aggravating endoplasmic reticulum stress through PERK/eIF2α arm of unfolded protein response and blocking autophagy.
Salubrinal enhances eIF2α phosphorylation and improves fertility in a mouse model of Classic Galactosemia.
LACC1 Required for NOD2-Induced, ER Stress-Mediated Innate Immune Outcomes in Human Macrophages and LACC1 Risk Variants Modulate These Outcomes.
Wang Z, Li M, Liu Y, Qiao Z, Bai T, Yang L, Liu B
Oncology reports 2021 Nov;46(5)
Oncology reports 2021 Nov;46(5)
Targeted Inhibition of P4HB Promotes Cell Sensitivity to Gemcitabine in Urothelial Carcinoma of the Bladder.
Wang X, Bai Y, Zhang F, Yang Y, Feng D, Li A, Yang Z, Li D, Tang Y, Wei X, Wei W, Han P
OncoTargets and therapy 2020;13:9543-9558
OncoTargets and therapy 2020;13:9543-9558
Towards Age-Related Anti-Inflammatory Therapy: Klotho Suppresses Activation of ER and Golgi Stress Response in Senescent Monocytes.
Mytych J, Sołek P, Będzińska A, Rusinek K, Warzybok A, Tabęcka-Łonczyńska A, Koziorowski M
Cells 2020 Jan 21;9(2)
Cells 2020 Jan 21;9(2)
Knockdown of cytokeratin 8 overcomes chemoresistance of chordoma cells by aggravating endoplasmic reticulum stress through PERK/eIF2α arm of unfolded protein response and blocking autophagy.
Wang D, Zhang P, Xu X, Wang J, Wang D, Peng P, Zheng C, Meng QJ, Yang L, Luo Z
Cell death & disease 2019 Nov 25;10(12):887
Cell death & disease 2019 Nov 25;10(12):887
Salubrinal enhances eIF2α phosphorylation and improves fertility in a mouse model of Classic Galactosemia.
Balakrishnan B, Siddiqi A, Mella J, Lupo A, Li E, Hollien J, Johnson J, Lai K
Biochimica et biophysica acta. Molecular basis of disease 2019 Nov 1;1865(11):165516
Biochimica et biophysica acta. Molecular basis of disease 2019 Nov 1;1865(11):165516
LACC1 Required for NOD2-Induced, ER Stress-Mediated Innate Immune Outcomes in Human Macrophages and LACC1 Risk Variants Modulate These Outcomes.
Huang C, Hedl M, Ranjan K, Abraham C
Cell reports 2019 Dec 24;29(13):4525-4539.e4
Cell reports 2019 Dec 24;29(13):4525-4539.e4
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of 453 and AD293 cells using an anti-EIF2AK3 polyclonal antibody (Product # PA5-40294).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3. DHA activates the UPRs in PLC cells in vitro . HepG2 and PLC/PRF/5 cells were treated with 40 and 25 uM DHA, respectively, for 1, 6, 12 or 24 h. (A and C) The protein expression levels of UPR-associated molecules were assessed by western blotting. Relative protein expression levels of indicated molecules are listed below the blots. (B and D) Total RNAs were isolated to analyze the formation of sXBP1 in PLC cells. DHA, dihydroartemisinin; PLC, primary liver cancer; UPR, unfolded protein response; p-, phosphorylated; t-, total; PERK, protein kinase R-like ER kinase; eIF2alpha, eukaryotic initiation factor 2alpha; IRE1alpha, inositol-requiring transmembrane kinase/endoribonuclease 1alpha; ATF, activating transcription factor.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 5 Knockdown of KRT8 followed by chemotherapy promoted apoptosis of chordoma cells through aggregating ER stress through PERK/eIF2alpha arm of UPR in vitro. Chordoma cell line CM319 and UCH1 were transfected with siKRT8 followed by treatment of doxorubicin (0.5 muM) or irinotecan (50 muM) for 24 h. a Splicing of XBP1 mRNA were evaluated by qRT-PCR. b Western blotting analysis and quantification of PERK, p-PERK, eIF2alpha, p-eIF2alpha, ATF6, and XBP1-s protein expression (p-PERK and p-eIF2alpha were normalized to PERK and eIF2alpha expression, respectively; ATF6 and XBP1-s were normalized to GAPDH expression; quantification data of ATF6 in ""Doxo 24 h"" group and ""Irino 24 h"" group was derived from the same data set in Fig. 2b ) ( n = 3, * p < 0.05 vs. indicated group, ** p < 0.01 vs. indicated group, NS: not statistically significant vs. indicated group, Con: control group, Doxo: doxorubicin-treated group, Irino: irinotecan-treated group. For all the above-mentioned statistical analyses, significance was determined by one-way ANOVA followed by Tukey's multiple comparisons test and the results were shown as mean +- SD).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 7 PERK inhibitor GSK2606414 decreased UPR activation and partially abolished chemosensitizing effect of siKRT8 but did not reverse the blockage of autophagy flux in vitro. Chordoma cell line CM319 and UCH1 were transfected with siKRT8 followed by treatment of doxorubicin (0.5 muM), irinotecan (50 muM), and GSK2606414 (2 muM) for 24 h. a Western blotting analysis and quantification of p-PERK, p-eIF2alpha, Cleaved PARP, SQSTM1, and LC3B protein expression (p-PERK and p-eIF2alpha were normalized to PERK and eIF2alpha expression, respectively; Cleaved PARP, LC3B, and SQSTM1 were normalized to GAPDH expression; quantification data of p-PERK, p-eIF2alpha, Cleaved PARP, SQSTM1, and LC3B in ""si + Doxo 24 h"" group and ""si + Irino 24 h"" group were derived from the same data set in Figs. 4b and 5b ). b Apoptosis of chordoma cells was determined by Annexin V-PE/PI staining measured by flow cytometry (quantification data of apoptosis in ""si + Doxo 24 h"" group and ""si + Irino 24 h"" group were derived from the same data set in Fig. 4d ) ( n = 3, * p < 0.05 vs. the indicated group, ** p < 0.01 vs. the indicated group, NS: not statistically significant vs. indicated group, Con: control group, Doxo: doxorubicin-treated group, Irino: irinotecan-treated group. For all the above-mentioned statistical analyses, significance was determined by one-way ANOVA followed by Tukey's multiple comparisons test and the results were shown as mean +- SD).