A01992-2

antibody from Boster Biological Technology

Targeting: EIF2AK3 PEK, PERK

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

A01992-2

Provider

Boster Biological Technology

Product name

Anti-PERK/EIF2AK3 Antibody Picoband™

Antibody type

Polyclonal

Description

Polyclonal antibody for PERK/EIF2AK3 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, FCM, Direct ELISA. Reactive species: Human. PERK/EIF2AK3 information: Subcellular Localization: Endoplasmic reticulum membrane; Single-pass type I membrane protein; Tissue Specificity: Ubiquitous. A high level expression is seen in secretory tissues.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of PERK using anti-PERK antibody (A01992-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,Lane 2: human COLO-320 whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: human SK-OV-3 whole cell lysates,Lane 5: Human A431 whole cell lysates,Lane 6: rat brain tissue lysates,Lane 7: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PERK antigen affinity purified polyclonal antibody (Catalog # A01992-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PERK at approximately 140KD. The expected band size for PERK is at 125KD.

Additional image