Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- 649402 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#649402, RRID:AB_10640071
- Product name
- Purified anti-PERK Phospho (Ser713)
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- Poly6494
- Vial size
- 400 µL
- Concentration
- 0.00 mg/mL
- Storage
- -20°C
- Handling
- Ambient RT
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Supportive validation
- Submitted by
- BioLegend (provider)
- Main image
- Experimental details
- 293E cells were transfected with PERK-FLAG construct. Cell lysates were then immunoprecipitated with L5 antibody. Proteins were then treated with calf intestinal alkaline phosphatase (CIP). The proteins without CIP treatment were used as the control. Samples were separated by electrophoresis, transferred to nitrocellulose and probed with anti-PERK phospho (Ser713) antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminenescent system.
- Conjugate
- Unconjugated
- Submitted by
- BioLegend (provider)
- Main image
- Experimental details
- Total cell lysate from 293T cells transfected with FLAG-PERK (lane 1) and FLAG-PERK S713A mutant (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-PERK Phospho (Ser713) antibody (clone Poly6494). Proteins were visualized using an HRP-conjugated anti-rabbit-IgG secondary and chemiluminescence detection.
- Conjugate
- Unconjugated