H00081579-M01

antibody from Abnova Corporation

Targeting: PLA2G12A PLA2G12

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00081579-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00081579-M01, RRID:AB_509381

Product name

PLA2G12A monoclonal antibody (M01), clone 1D11

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant PLA2G12A.

Antigen sequence

PLFGVHLNIGIPSLTKCCNQHDRCYETCGKSKNDC
DEEFQYCLSKICRDVQKTLGLTQHVQACETTVELL
FDSVIHLGCKPYLDSQRAACRCHYEEKTD


Isotype

IgG

Antibody clone number

1D11

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Expression of group XIIA phospholipase A2 in human digestive organs.

Peuravuori H, Kollanus S, Nevalainen TJ
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2014 Dec;122(12):1171-7

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of PLA2G12A expression in transfected 293T cell line by PLA2G12A monoclonal antibody (M01), clone 1D11.Lane 1: PLA2G12A transfected lysate(21.1 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged PLA2G12A is approximately 0.3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol