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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- PA5-49382 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CLN3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Predicted to react with monkey based on sequence homology.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.4 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Gene correction of the CLN3 c.175G>A variant in patient-derived induced pluripotent stem cells prevents pathological changes in retinal organoids.
Zhang X, Zhang D, Thompson JA, Chen SC, Huang Z, Jennings L, McLaren TL, Lamey TM, De Roach JN, Chen FK, McLenachan S
Molecular genetics & genomic medicine 2021 Mar;9(3):e1601
Molecular genetics & genomic medicine 2021 Mar;9(3):e1601
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CLN3 in lysates from A431, KG-1, SH-SY5Y cell line, mouse kidney, mouse liver tissue (from left to right). Samples were probed with CLN3 Antibody (Center) (Product # PA5-49382) diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CLN3 in lysates from A431, KG-1, SH-SY5Y cell line, mouse kidney, mouse liver tissue (from left to right). Samples were probed with CLN3 Antibody (Center) (Product # PA5-49382) diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of CLN3 in HeLa Cells. Cells were incubated with a CLN3 Antibody (Center) (green) (Product # PA5-49382) at a at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody. Isotype control of rabbit IgG (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 2 FIGURE Protein samples from undifferentiated iPSC, 4-week CM, and 5-week NRO cultures were analysed by western blotting using an anti-CLN3 antibody and an anti-GAPDH antibody. LEIi004-A patient (P) iPSC and CM expressed lower levels of CLN3 protein than gene-corrected LEIi004-A-1 patient (GC) iPSC and CM. LEIi004-A-1 iPSC expressed similar levels of CLN3 protein as a control iPSC line (Con). Similar levels of CLN3 protein expression were detected in NRO from LEIi004-A and LEIi004-A-1
