16534-1-AP

antibody from Proteintech Group

Targeting: CTHRC1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

16534-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#16534-1-AP, RRID:AB_2087511

Product name

CTHRC1 antibody

Antibody type

Polyclonal

Description

KD/KO validated CTHRC1 antibody (Cat. #16534-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Elevated lactate production exacerbates PM2.5-induced pulmonary fibrosis by stabilizing TGF-β1.

Liu Z, Liu W, Wei H, Ping Y, Yu Z, Dong Z, Ren J, Zhang S, Liu S
Journal of advanced research 2026 May;83:299-314

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SOX4 is a transcriptional activator for CTHRC1 in lung fibroblast activation.

Huang Y, Wu L, Feng D, Zhang C, Zhuang L, Jin R, Zhou G
Gene 2026 Jul 10;998:150171

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Pathologic Wall Shear Stress in the Trachea Decreases Epithelial Differentiation and Increases Fibrosis.

Dharmadhikari S, Calyeca J, Liu L, Wu Z, Schneller A, Hussein Z, Tan ZH, Breuer CK, Reynolds SD, Zhao K, Chiang T
American journal of respiratory cell and molecular biology 2026 Jan 23;

---

Deep-learning-based de novo discovery and design of therapeutics that reverse disease-associated transcriptional phenotypes.

Xing J, Tan M, Leshchiner D, Sun M, Abdelgied M, Huang L, Paithankar S, Uhl K, Shankar R, Lisabeth E, Aleiwi B, Jager T, Lawson C, Chen R, Giletto M, Girgis R, Neubig RR, So S, Ellsworth E, Li X, Chua MS, Zhou J, Chen B
Cell 2026 Apr 30;189(9):2556-2572.e19

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Analysis of the anti-fibrotic potential of a JAK inhibitor in a bleomycin-induced pulmonary fibrosis model.

Nishimura H, Kawano H, Sato S, Imakura T, Yamashita Y, Murakami K, Haji K, Bando H, Naito N, Kagawa K, Isomura Y, Koyama K, Ogawa H, Nishimura Y, Nishioka Y
The journal of medical investigation : JMI 2025;72(3.4):298-307

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Exploring the wound-healing mechanism of Cayratia japonica extract: A combined experimental and network pharmacology study.

Cheng M, Hou Y, Chen Q, Ge S, Chen C, Zheng X, Zhang C
Journal of ethnopharmacology 2025 May 12;347:119810

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Single-cell and spatial transcriptome profiling reveal CTHRC1+ fibroblasts promote EMT through WNT5A signaling in colorectal cancer.

Lu Y, Chen Y, Wang Z, Shen H, Xu L, Huang C, Tong Y, Shao Y, Zhang H, Fu Z
Journal of translational medicine 2025 Mar 6;23(1):282

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Orchestrated response from heterogenous fibroblast subsets contributes to repair from surgery-induced stress after airway reconstruction.

Calyeca J, Hussein Z, Tan ZH, Liu L, Dharmadhikari S, Shontz KM, Vetter TA, Breuer CK, Reynolds SD, Chiang T
JCI insight 2025 Jan 21;10(5)

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CTHRC1 serves as an indicator in biliary atresia for evaluating the stage of liver fibrosis and predicting prognosis.

Ding Z, Zhang R, Zhu W, Lu Y, Zhu Z, Xie H, Tang W
Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver 2025 Feb;57(2):385-393

---

Emergence of inflammatory fibroblasts with aging in Hermansky-Pudlak syndrome associated pulmonary fibrosis.

Banaschewski BJH, Michki SN, Sitaraman S, Pan R, Wang JY, Stewart D, Goldy MK, Lin SM, Cantu E, Katzen JB, Basil MC, Emtiazjoo AM, Todd JL, Gokey JJ, Kropski JA, Frank DB, Zepp JA, Young LR
Communications biology 2025 Feb 22;8(1):284

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Senescent fibroblasts secrete CTHRC1 to promote cancer stemness in hepatocellular carcinoma.

Huang H, Peng W, Zhou Q, Zhao Y, Liu L, Cui H, Liang J, Cao M, Chen W, Wang R, Chen S, Xiong S, Cheng B, Bai S
Cell communication and signaling : CCS 2025 Aug 25;23(1):379

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Expression and role of CTHRC1 in inflammatory bowel disease in children.

Tang H, Gao X, Wu Z, Chen J, Chen L, Du X
Cytotechnology 2025 Apr;77(2):44

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Expression, Prognostic Value, and Biological Function of CTHRC1 in Different Types of Gliomas: A Bioinformatic Analysis and Experiment Validation.

Shi X, Zeng X, Jiao R, Yang Y, Du X, Qian J, Liu J, Chen G
Clinical Medicine Insights. Oncology 2024;18:11795549241260576

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Cancer-Associated Fibroblasts Expressing Sulfatase 1 Facilitate VEGFA-Dependent Microenvironmental Remodeling to Support Colorectal Cancer.

Wang H, Chen J, Chen X, Liu Y, Wang J, Meng Q, Wang H, He Y, Song Y, Li J, Ju Z, Xiao P, Qian J, Song Z
Cancer research 2024 Oct 15;84(20):3371-3387

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CTHRC1 is associated with BRAF(V600E) mutation and correlates with prognosis, immune cell infiltration, and drug resistance in colon cancer, thyroid cancer, and melanoma.

Zhang R, Wang Z, Wang H, Li L, Dong L, Ding L, Li Q, Zhu L, Zhang T, Zhu Y, Ding K
Biomolecules & biomedicine 2024 Dec 11;25(1):42-61

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Exosomal CTHRC1 from cancer-associated fibroblasts facilitates endometrial cancer progression via ITGB3/FAK signaling pathway.

Bian Y, Chang X, Hu X, Li B, Song Y, Hu Z, Wang K, Wan X, Lu W
Heliyon 2024 Aug 30;10(16):e35727

---

E3 ubiquitin ligase NEDD4L inhibits epithelial-mesenchymal transition by suppressing the β-catenin/HIF-1α positive feedback loop in chronic rhinosinusitis with nasal polyps.

Chen SY, Liu PQ, Qin DX, Lv H, Zhou HQ, Xu Y
Acta pharmacologica Sinica 2024 Apr;45(4):831-843

---

Collagen Triple Helix Repeat Containing 1 Deficiency Protects Against Airway Remodeling and Inflammation in Asthma Models In Vivo and In Vitro.

Feng Y, Hu J, Liu F, Shang Y
Inflammation 2023 Jun;46(3):925-940

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CTHRC1 is associated with immune cell infiltration and functions as an adverse marker for prognosis in head and neck squamous cell carcinoma.

Zhong R, Yang M, Zhu R, Zhang J, Wang L
Oncology letters 2023 Apr;25(4):133

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The molecular mechanisms of CTHRC1 in gastric cancer by integrating TCGA, GEO and GSA datasets.

Zhao L, Wang W, Niu P, Luan X, Zhao D, Chen Y
Frontiers in genetics 2022;13:900124

---

Single-cell landscape reveals active cell subtypes and their interaction in the tumor microenvironment of gastric cancer.

Li Y, Hu X, Lin R, Zhou G, Zhao L, Zhao D, Zhang Y, Li W, Zhang Y, Ma P, Ren H, Liao X, Niu P, Wang T, Zhang X, Wang W, Gao R, Li Q, Church G, He J, Chen Y
Theranostics 2022;12(8):3818-3833

---

Fulminant lung fibrosis in non-resolvable COVID-19 requiring transplantation.

Jyothula SSK, Peters A, Liang Y, Bi W, Shivshankar P, Yau S, Garcha PS, Yuan X, Akkanti B, Collum S, Wareing N, Thandavarayan RA, Poli de Frias F, Rosas IO, Zhao B, Buja LM, Eltzschig HK, Huang HJ, Karmouty-Quintana H
EBioMedicine 2022 Dec;86:104351

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Human Placental Endothelial Cell and Trophoblast Heterogeneity and Differentiation Revealed by Single-Cell RNA Sequencing.

Li H, Peng H, Hong W, Wei Y, Tian H, Huang X, Jia L, Zheng J, Duan T, He Q, Wang K
Cells 2022 Dec 25;12(1)

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MicroRNA Hsa-Let-7b Regulates the Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Targeting CTHRC1.

Fu L, Li N, Ye Y, Ye X, Xiao T, Wu X, Ma Y, Yu J
Stem cells international 2021;2021:5791181

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NEDD4L-induced β-catenin ubiquitination suppresses the formation and progression of interstitial pulmonary fibrosis via inhibiting the CTHRC1/HIF-1α axis.

Chen L, Yang Y, Yan H, Peng X, Zou J
International journal of biological sciences 2021;17(13):3320-3330

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Hyperdense Pancreatic Ductal Adenocarcinoma: Clinical Characteristics and Proteomic Landscape.

Xu H, Hua J, Meng Q, Wang X, Xu J, Wang W, Zhang B, Liu J, Liang C, Yu X, Shi S
Frontiers in oncology 2021;11:640820

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miR-509-3p Suppresses Migration, Invasion, and Epithelial- Mesenchymal Transition in Melanoma Cells by Targeting Collagen Triple Helix Repeat Containing 1.

Yuan K, Sun Y, Ji Y
Balkan medical journal 2021 May;38(3):177-182

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Identification of Overexpressed Genes in Malignant Pleural Mesothelioma.

Morani F, Bisceglia L, Rosini G, Mutti L, Melaiu O, Landi S, Gemignani F
International journal of molecular sciences 2021 Mar 8;22(5)

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CTHRC1 in Ovarian Cancer Promotes M2-Like Polarization of Tumor-Associated Macrophages via Regulation of the STAT6 Signaling Pathway.

Bai Y, Yin K, Su T, Ji F, Zhang S
OncoTargets and therapy 2020;13:5743-5753

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Cyclovirobuxine D inhibits colorectal cancer tumorigenesis via the CTHRC1‑AKT/ERK‑Snail signaling pathway.

Jiang F, Chen Y, Ren S, Li Z, Sun K, Xing Y, Zhu Y, Piao D
International journal of oncology 2020 Jul;57(1):183-196

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CTHRC1 affects malignant tumor cell behavior and is regulated by miR-30e-5p in human prostate cancer.

Ma Z, Chao F, Wang S, Song Z, Zhuo Z, Zhang J, Xu G, Chen G
Biochemical and biophysical research communications 2020 Apr 30;525(2):418-424

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CTHRC1 May Serve As A Prognostic Biomarker For Hepatocellular Carcinoma.

Zhou H, Su L, Liu C, Li B, Li H, Xie Y, Sun D
OncoTargets and therapy 2019;12:7823-7831

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Pirfenidone attenuates lung fibrotic fibroblast responses to transforming growth factor-β1.

Jin J, Togo S, Kadoya K, Tulafu M, Namba Y, Iwai M, Watanabe J, Nagahama K, Okabe T, Hidayat M, Kodama Y, Kitamura H, Ogura T, Kitamura N, Ikeo K, Sasaki S, Tominaga S, Takahashi K
Respiratory research 2019 Jun 11;20(1):119

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CTHRC1 promotes M2-like macrophage recruitment and myometrial invasion in endometrial carcinoma by integrin-Akt signaling pathway.

Li LY, Yin KM, Bai YH, Zhang ZG, Di W, Zhang S
Clinical & experimental metastasis 2019 Aug;36(4):351-363

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CTHRC1 mediates IL‑1β‑induced apoptosis in chondrocytes via JNK1/2 signaling.

Zhang Q, Yin ZS, Zhang FW, Cao K, Sun HY
International journal of molecular medicine 2018 Apr;41(4):2270-2278

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Collagen triple helix repeat containing 1 (CTHRC1) activates Integrin β3/FAK signaling and promotes metastasis in ovarian cancer.

Guo B, Yan H, Li L, Yin K, Ji F, Zhang S
Journal of ovarian research 2017 Oct 11;10(1):69

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Lentivirus-Mediated Knockdown of CTHRC1 Inhibits Osteosarcoma Cell Proliferation and Migration.

Sang W, Zhu L, Ma J, Lu H, Wang C
Cancer biotherapy & radiopharmaceuticals 2016 Apr;31(3):91-8

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SP1-mediated microRNA-520d-5p suppresses tumor growth and metastasis in colorectal cancer by targeting CTHRC1.

Yan L, Yu J, Tan F, Ye GT, Shen ZY, Liu H, Zhang Y, Wang JF, Zhu XJ, Li GX
American journal of cancer research 2015;5(4):1447-59

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MiR-9 inhibits Schwann cell migration by targeting Cthrc1 following sciatic nerve injury.

Zhou S, Gao R, Hu W, Qian T, Wang N, Ding G, Ding F, Yu B, Gu X
Journal of cell science 2014 Mar 1;127(Pt 5):967-76

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

A375 cells were subjected to SDS PAGE followed by western blot with 16534-1-AP(CTHRC1 antibody) at dilution of 1:800

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human breast cancer using 16534-1-AP(CTHRC1 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The CTHRC1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human CTHRC1. This antibody recognizes human,mouse,rat antigen. The CTHRC1 antibody has been validated for the following applications: ELISA, WB, IHC analysis.

Appendix

16534-1-AP.pdf

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