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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- PA5-49638 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CTHRC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Dynamic Interstitial Cell Response during Myocardial Infarction Predicts Resilience to Rupture in Genetically Diverse Mice.
Forte E, Skelly DA, Chen M, Daigle S, Morelli KA, Hon O, Philip VM, Costa MW, Rosenthal NA, Furtado MB
Cell reports 2020 Mar 3;30(9):3149-3163.e6
Cell reports 2020 Mar 3;30(9):3149-3163.e6
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CTHRC1 in A375 cell line lysates (35 µg/lane). CTHRC1 (arrow) was detected using a CTHRC1 Antibody (N-term) (Product # PA5-49638).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CTHRC1 in A375 cell line lysates (35 µg/lane). CTHRC1 (arrow) was detected using a CTHRC1 Antibody (N-term) (Product # PA5-49638).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CTHRC1 (arrow)in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CTHRC1 gene. Samples were probed with a rabbit polyclonal CTHRC1 Antibody (N-term) (Product # PA5-49638).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CTHRC1 in Formalin-fixed and paraffin-embedded human skin tissue. Samples were probed with a CTHRC1 Antibody (N-term) (Product # PA5-49638) followed by peroxidase-conjugation to the secondary antibody, and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of CTHRC1 in A375 cells (right histogram) compared to a negative control cell (left histogram). Samples were probed with a CTHRC1 Antibody (N-term) (Product # PA5-49638). FITC-conjugated goat anti-rabbit secondary antibodies were used for the analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Contribution of Myofibroblasts ( Myofb ), Matrifibrocytes ( MFCs ), and Late Response Fibroblasts ( LR ) to Ventricular Remodeling Post-MI (A) knn SPRING visualization of the Myofb cluster identified from analysis of the stromal aggregate across 7 time points (PC24, resolution 0.5), colored by time and by the expression of early Myofb marker genes ( Acta2 , Tnc , Cthrc1 , light blue) and MFC marker genes ( Sfrp2 , Clu , Ecrg4 , Comp , Wisp2 , Thsb4) . (B and C) Confocal imaging of adult hearts from Col1a1 eGFP mice, used as a genetic marker for stromal cells. (B) Myofb markers CTHRC1 (red) and ACTA2 (white) and (C) MFC markers SFRP2 (red) and CLU (white) at different time points post-MI. (D) qPCR validation of additional Myofb (light blue), MFC (dark blue), and LR (olive green) markers on live nucleated stromal cells (PI - , DRAQ5 + , EGFP + ) sorted from Col1a1 eGFP transgenic hearts. Scar or distal areas were dissected and separately processed for sorting from control (n = 3) or d5 (n = 3) and d14 (n = 3) post-MI ventricular tissue. Data are summarized as box and whisker plots, indicating the median value (black bar inside box), 25th and 75th percentiles (bottom and top of box, respectively), and minimum and maximum values (bottom and top whisker, respectively). Statistical significance ( * p < 0.05, zetap < 0.001, #p < 0.0001) was calculated per each gene by two-tailed unequal variance Student's t test between sample (distal d5, distal d14
