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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
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Validation data
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- Product number
- 22010-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PHF20 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MTKHPPNRR GISFEVGAQL EARDRLKNWY PAHIEDIDYE EGKVLIHFKR WNHRYDEWFC WDSPYLRPLE KIQLRKEGLH EEDGSSEFQI NEQVLACWSD CRFYPAKVTA VNKDGTYTVK FYDGVVQTVK HIHVKAFSKD QNIVGNARPK ETDHKSLSSS PDKREKFKEQ RKATVNVKKD KEDKPLKTEK RPKQPDKEGK LICSEKGKVS EKSLPKNEKE DKENISENDR EYSGDAQVDK KPENDIVKSP QENLREPKRK RGRPPSIAPT AVDSNSQTLQ PITLELRRRK ISKGCEVPLK RPRLDKNSSQ EKSKNYSENT DKDLSRRRSS RLSTNGTHEI LDPDLVVSDL V (1-350 aa encoded by BC150178)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.27 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse testis tissue were subjected to SDS PAGE followed by western blot with 22010-1-AP (PHF20 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 22010-1-AP ( PHF20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 22010-1-AP (PHF20 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 22010-1-AP (PHF20 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 22010-1-AP (PHF20 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
