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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunoprecipitation [1]
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Validation data
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- Product number
- MA5-55830 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LRRN1 Monoclonal Antibody (K16288_6D3)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Sequence of this protein is as follows: ECPQLCVCEI RPWFTPQSTY REATTVDCND LRLTRIPSNL SSDTQVLLLQ SNNIAKTVDE LQQLFNLTEL DFSQNNFTNI KEVGLANLTQ LTTLHLEENQ ITEMTDYCLQ DLSNLQELYI NHNQISTISA HAFAGLKNLL RLHLNSNKLK VIDSRWFDST PNLEILMIGE NPVIGILDMN FKPLANLRSL VLAGMYLTDI PGNALVGLDS LESLSFYDNK LVKVPQLALQ KVPNLKFLDL NKNPIHKIQE GDFKNMLRLK ELGINNMGEL VSVDRYALDN LPELTKLEAT NNPKLSYIHR LAFRSVPALE SLMLNNNALN AIYQKTVESL PNLREISIHS NPLRCDCVIH WINSNKTNIR FMEPLSMFCA MPPEYKGHQV KEVLIQDSSE QCLPMISHDS FPNRLNVDIG TTVFLDCRAM AEPEPEIYWV TPIGNKITVE TLSDKYKLSS EGTLEISNIQ IEDSGRYTCV AQNVQGADTR VATIKVNGTL LDGTQVLKIY VKQTESHSIL VSWKVNSNVM TSNLKWSSAT MKIDNPHITY TARVPVDVHE YNLTHLQPST DYEVCLTVSN IHQQTQKSCV NVTTKNAAFA VDISDQETST
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- K16288_6D3
- Vial size
- 50 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of LRRN1 in 200 µg of SH-SY5Y lysate. Samples are as follows: Lane 1: SH-SY5Y lysate, Lane 2: LRRN1 immunoprecipitated from SH-SY5Y lysate, Lane3: The same as Lane 2 but KT82 was used as IgG isotype control antibody. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE, blotted onto nitrocellulose membrane, and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody. The isotype control antibody was KT82. Incubation of samples with LRRN1 monoclonal antibody (Product # MA5-55830) at a dilution of 2.5 µg was used.
