Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF4990 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse LRRN1/NLRR-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human LRRN1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human NLRR-3 is observed.
- Reactivity
- Human, Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
AAH34947
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Molecular Logic of Spinocerebellar Tract Neuron Diversity and Connectivity.
Neuronal leucine-rich repeat 1 negatively regulates anaplastic lymphoma kinase in neuroblastoma.
Baek M, Menon V, Jessell TM, Hantman AW, Dasen JS
Cell reports 2019 May 28;27(9):2620-2635.e4
Cell reports 2019 May 28;27(9):2620-2635.e4
Neuronal leucine-rich repeat 1 negatively regulates anaplastic lymphoma kinase in neuroblastoma.
Satoh S, Takatori A, Ogura A, Kohashi K, Souzaki R, Kinoshita Y, Taguchi T, Hossain MS, Ohira M, Nakamura Y, Nakagawara A
Scientific reports 2016 Sep 8;6:32682
Scientific reports 2016 Sep 8;6:32682
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- LRRN1/NLRR-1 in Embryonic Mouse Somites. LRRN1/NLRR-1 was detected in immersion fixed frozen sections of embryonic mouse somites (E9.5) using 10 µg/mL Sheep Anti-Human LRRN1/NLRR-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4990) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.