PA5-112470

antibody from Invitrogen Antibodies

Targeting: MACROH2A1 H2AFY, macroH2A1.2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-112470

Provider

Invitrogen Antibodies

Product name

Acetyl-Histone Macro-H2A.1 (Lys36) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.23 mg/mL

Storage

-20°C or -80°C if preferred

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Acetyl Histone Macro-H2A.1 (Lys36) using a polyclonal antibody (Product # PA5-112470) at a dilution of 2 µg/mL. A goat anti-rabbit polyclonal secondary antibody at a dilution of 1:50,000 was used for detection in HepG2 whole cell lysate, and A549 whole cell lysate (both treated with 30 mM sodium butyrate for 4 hours). Observed band: 15 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Acetyl Histone Macro-H2A.1 (Lys36) in Hela cells using a Acetyl Histone Macro-H2A.1 (Lys36) polyclonal antibody (Product # PA5-112470) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Acetyl Histone Macro-H2A.1 (Lys36) in Hela cells using a Acetyl Histone Macro-H2A.1 (Lys36) polyclonal antibody (Product # PA5-112470) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemichal analysis of Acetyl Histone Macro-H2A.1 (Lys36) in Hela cells using a Polyclonal antibody (Product # PA5-112470) at a dilution of 1:100. An Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) secondary antibdoy was used.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemichal analysis of Acetyl Histone Macro-H2A.1 (Lys36) in Hela cells using a Polyclonal antibody (Product # PA5-112470) at a dilution of 1:100. An Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) secondary antibdoy was used.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Acetyl Histone Macro-H2A.1 (Lys36) in Hela cells using a Acetyl Histone Macro-H2A.1 (Lys36) polyclonal antibody (Product # PA5-112470) at a dilution of 1:100.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Chromatin Immunoprecipitation analysis of Acetyl Histone Macro-H2A.1 (Lys36) in 4*10^6 Hela cells using a Acetyl Histone Macro-H2A.1 (Lys36) polyclonal antibody (Product #PA5-112470). Hela cells were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8 µg anti-HIST1H2AG or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Chromatin Immunoprecipitation analysis of Acetyl Histone Macro-H2A.1 (Lys36) in 4*10^6 Hela cells using a Acetyl Histone Macro-H2A.1 (Lys36) polyclonal antibody (Product #PA5-112470). Hela cells were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8 µg anti-HIST1H2AG or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter.