16139-1-AP

antibody from Proteintech Group

Targeting: MRPS18B C6orf14, HSPC183, MRPS18-2, PTD017

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

16139-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#16139-1-AP, RRID:AB_2146368

Product name

MRPS18B antibody

Antibody type

Polyclonal

Description

MRPS18B antibody (Cat. #16139-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

A kinetic dichotomy between mitochondrial and nuclear gene expression processes.

McShane E, Couvillion M, Ietswaart R, Prakash G, Smalec BM, Soto I, Baxter-Koenigs AR, Choquet K, Churchman LS
Molecular cell 2024 Apr 18;84(8):1541-1555.e11

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Cross-link assisted spatial proteomics to map sub-organelle proteomes and membrane protein topologies.

Zhu Y, Akkaya KC, Ruta J, Yokoyama N, Wang C, Ruwolt M, Lima DB, Lehmann M, Liu F
Nature communications 2024 Apr 17;15(1):3290

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Human Mitoribosome Profiling: A Re-engineered Approach Tailored to Study Mitochondrial Translation.

Soto I, Couvillion M, Stirling Churchman L
Methods in molecular biology (Clifton, N.J.) 2023;2661:257-280

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A kinetic dichotomy between mitochondrial and nuclear gene expression drives OXPHOS biogenesis.

McShane E, Couvillion M, Ietswaart R, Prakash G, Smalec BM, Soto I, Baxter-Koenigs AR, Choquet K, Churchman LS
bioRxiv : the preprint server for biology 2023 Sep 6;

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Lysosomal cystine governs ferroptosis sensitivity in cancer via cysteine stress response.

Swanda RV, Ji Q, Wu X, Yan J, Dong L, Mao Y, Uematsu S, Dong Y, Qian SB
Molecular cell 2023 Sep 21;83(18):3347-3359.e9

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Let's make it clear: systematic exploration of mitochondrial DNA- and RNA-protein complexes by complexome profiling.

Potter A, Cabrera-Orefice A, Spelbrink JN
Nucleic acids research 2023 Oct 27;51(19):10619-10641

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Regulators of mitonuclear balance link mitochondrial metabolism to mtDNA expression.

Kramer NJ, Prakash G, Isaac RS, Choquet K, Soto I, Petrova B, Merens HE, Kanarek N, Churchman LS
Nature cell biology 2023 Nov;25(11):1575-1589

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Balanced mitochondrial and cytosolic translatomes underlie the biogenesis of human respiratory complexes.

Soto I, Couvillion M, Hansen KG, McShane E, Moran JC, Barrientos A, Churchman LS
Genome biology 2022 Aug 9;23(1):170

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Mitochondrial microproteins link metabolic cues to respiratory chain biogenesis.

Liang C, Zhang S, Robinson D, Ploeg MV, Wilson R, Nah J, Taylor D, Beh S, Lim R, Sun L, Muoio DM, Stroud DA, Ho L
Cell reports 2022 Aug 16;40(7):111204

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Ovarian carcinoma immunoreactive antigen-like protein 2 (OCIAD2) is a novel complex III-specific assembly factor in mitochondria.

Chojnacka KJ, Elancheliyan P, Mussulini BHM, Mohanraj K, Callegari S, Gosk A, Banach T, Góral T, Szczepanowska K, Rehling P, Serwa RA, Chacinska A
Molecular biology of the cell 2022 Apr 1;33(4):ar29

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Messenger RNA delivery to mitoribosomes - hints from a bacterial toxin.

Bruni F, Proctor-Kent Y, Lightowlers RN, Chrzanowska-Lightowlers ZM
The FEBS journal 2021 Jan;288(2):437-451

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A deafness-associated tRNA mutation caused pleiotropic effects on the m1G37 modification, processing, stability and aminoacylation of tRNAIle and mitochondrial translation.

Meng F, Zhou M, Xiao Y, Mao X, Zheng J, Lin J, Lin T, Ye Z, Cang X, Fu Y, Wang M, Guan MX
Nucleic acids research 2021 Jan 25;49(2):1075-1093

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Defining the interactome of the human mitochondrial ribosome identifies SMIM4 and TMEM223 as respiratory chain assembly factors.

Dennerlein S, Poerschke S, Oeljeklaus S, Wang C, Richter-Dennerlein R, Sattmann J, Bauermeister D, Hanitsch E, Stoldt S, Langer T, Jakobs S, Warscheid B, Rehling P
eLife 2021 Dec 31;10

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FMRP Control of Ribosome Translocation Promotes Chromatin Modifications and Alternative Splicing of Neuronal Genes Linked to Autism.

Shah S, Molinaro G, Liu B, Wang R, Huber KM, Richter JD
Cell reports 2020 Mar 31;30(13):4459-4472.e6

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YY1 control of mitochondrial-related genes does not account for regulation of immunoglobulin class switch recombination in mice.

Nandi S, Liang G, Sindhava V, Angireddy R, Basu A, Banerjee S, Hodawadekar S, Zhang Y, Avadhani NG, Sen R, Atchison ML
European journal of immunology 2020 Jun;50(6):822-838

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RCC1L (WBSCR16) isoforms coordinate mitochondrial ribosome assembly through their interaction with GTPases.

Reyes A, Favia P, Vidoni S, Petruzzella V, Zeviani M
PLoS genetics 2020 Jul;16(7):e1008923

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Inducible Germline IgMs Bridge Trypanosome Lytic Factor Assembly and Parasite Recognition.

Verdi J, Zipkin R, Hillman E, Gertsch RA, Pangburn SJ, Thomson R, Papavasiliou N, Sternberg J, Raper J
Cell host & microbe 2020 Jul 8;28(1):79-88.e4

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MITRAC15/COA1 promotes mitochondrial translation in a ND2 ribosome-nascent chain complex.

Wang C, Richter-Dennerlein R, Pacheu-Grau D, Liu F, Zhu Y, Dennerlein S, Rehling P
EMBO reports 2020 Jan 7;21(1):e48833

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Expression pattern of MRPS18 family genes in malignantly transformed b-cells.

Kovalevska L, Kashuba E
Experimental oncology 2020 Dec;42(4):295-299

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TRMT2B is responsible for both tRNA and rRNA m(5)U-methylation in human mitochondria.

Powell CA, Minczuk M
RNA biology 2020 Apr;17(4):451-462

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RNase H1 Regulates Mitochondrial Transcription and Translation via the Degradation of 7S RNA.

Reyes A, Rusecka J, Tońska K, Zeviani M
Frontiers in genetics 2019;10:1393

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METTL15 introduces N4-methylcytidine into human mitochondrial 12S rRNA and is required for mitoribosome biogenesis.

Van Haute L, Hendrick AG, D'Souza AR, Powell CA, Rebelo-Guiomar P, Harbour ME, Ding S, Fearnley IM, Andrews B, Minczuk M
Nucleic acids research 2019 Nov 4;47(19):10267-10281

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Mutations in the MRPS28 gene encoding the small mitoribosomal subunit protein bS1m in a patient with intrauterine growth retardation, craniofacial dysmorphism and multisystemic involvement.

Pulman J, Ruzzenente B, Bianchi L, Rio M, Boddaert N, Munnich A, Rötig A, Metodiev MD
Human molecular genetics 2019 May 1;28(9):1445-1462

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Mitochondrial stress response triggered by defects in protein synthesis quality control.

Richter U, Ng KY, Suomi F, Marttinen P, Turunen T, Jackson C, Suomalainen A, Vihinen H, Jokitalo E, Nyman TA, Isokallio MA, Stewart JB, Mancini C, Brusco A, Seneca S, Lombès A, Taylor RW, Battersby BJ
Life science alliance 2019 Feb;2(1)

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A variant in MRPS14 (uS14m) causes perinatal hypertrophic cardiomyopathy with neonatal lactic acidosis, growth retardation, dysmorphic features and neurological involvement.

Jackson CB, Huemer M, Bolognini R, Martin F, Szinnai G, Donner BC, Richter U, Battersby BJ, Nuoffer JM, Suomalainen A, Schaller A
Human molecular genetics 2019 Feb 15;28(4):639-649

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The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.

Lavdovskaia E, Kolander E, Steube E, Mai MM, Urlaub H, Richter-Dennerlein R
Nucleic acids research 2018 Sep 19;46(16):8471-8482

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LONP1 Is Required for Maturation of a Subset of Mitochondrial Proteins, and Its Loss Elicits an Integrated Stress Response.

Zurita Rendón O, Shoubridge EA
Molecular and cellular biology 2018 Oct 15;38(20)

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The MRPS18-2 protein levels correlate with prostate tumor progression and it induces CXCR4-dependent migration of cancer cells.

Mushtaq M, Jensen L, Davidsson S, Grygoruk OV, Andrén O, Kashuba V, Kashuba E
Scientific reports 2018 Feb 2;8(1):2268

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Inhibition of mitochondrial translation in fibroblasts from a patient expressing the KARS p.(Pro228Leu) variant and presenting with sensorineural deafness, developmental delay, and lactic acidosis.

Ruzzenente B, Assouline Z, Barcia G, Rio M, Boddaert N, Munnich A, Rötig A, Metodiev MD
Human mutation 2018 Dec;39(12):2047-2059

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The human RNA-binding protein RBFA promotes the maturation of the mitochondrial ribosome.

Rozanska A, Richter-Dennerlein R, Rorbach J, Gao F, Lewis RJ, Chrzanowska-Lightowlers ZM, Lightowlers RN
The Biochemical journal 2017 Jun 13;474(13):2145-2158

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A pseudouridine synthase module is essential for mitochondrial protein synthesis and cell viability.

Antonicka H, Choquet K, Lin ZY, Gingras AC, Kleinman CL, Shoubridge EA
EMBO reports 2017 Jan;18(1):28-38

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Biallelic Mutations in MRPS34 Lead to Instability of the Small Mitoribosomal Subunit and Leigh Syndrome.

Lake NJ, Webb BD, Stroud DA, Richman TR, Ruzzenente B, Compton AG, Mountford HS, Pulman J, Zangarelli C, Rio M, Boddaert N, Assouline Z, Sherpa MD, Schadt EE, Houten SM, Byrnes J, McCormick EM, Zolkipli-Cunningham Z, Haude K, Zhang Z, Retterer K, Bai R, Calvo SE, Mootha VK, Christodoulou J, Rötig A, Filipovska A, Cristian I, Falk MJ, Metodiev MD, Thorburn DR
American journal of human genetics 2017 Aug 3;101(2):239-254

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Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein.

Richter-Dennerlein R, Oeljeklaus S, Lorenzi I, Ronsör C, Bareth B, Schendzielorz AB, Wang C, Warscheid B, Rehling P, Dennerlein S
Cell 2016 Oct 6;167(2):471-483.e10

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Pathological ribonuclease H1 causes R-loop depletion and aberrant DNA segregation in mitochondria.

Akman G, Desai R, Bailey LJ, Yasukawa T, Dalla Rosa I, Durigon R, Holmes JB, Moss CF, Mennuni M, Houlden H, Crouch RJ, Hanna MG, Pitceathly RD, Spinazzola A, Holt IJ
Proceedings of the National Academy of Sciences of the United States of America 2016 Jul 26;113(30):E4276-85

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Mitochondrial Ribosomal Protein L12 Is Required for POLRMT Stability and Exists as Two Forms Generated by Alternative Proteolysis during Import.

Nouws J, Goswami AV, Bestwick M, McCann BJ, Surovtseva YV, Shadel GS
The Journal of biological chemistry 2016 Jan 8;291(2):989-97

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Mitochondrial ribosomal protein S18-2 is highly expressed in endometrial cancers along with free E2F1.

Mints M, Mushtaq M, Iurchenko N, Kovalevska L, Stip MC, Budnikova D, Andersson S, Polischuk L, Buchynska L, Kashuba E
Oncotarget 2016 Apr 19;7(16):22150-8

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Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness.

Richter U, Lahtinen T, Marttinen P, Suomi F, Battersby BJ
The Journal of cell biology 2015 Oct 26;211(2):373-89

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Translational control of the cytosolic stress response by mitochondrial ribosomal protein L18.

Zhang X, Gao X, Coots RA, Conn CS, Liu B, Qian SB
Nature structural & molecular biology 2015 May;22(5):404-10

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Mitochondrial RNA Granules Are Centers for Posttranscriptional RNA Processing and Ribosome Biogenesis.

Antonicka H, Shoubridge EA
Cell reports 2015 Feb 17;10(6):920-932

---

Amino acid starvation has opposite effects on mitochondrial and cytosolic protein synthesis.

Johnson MA, Vidoni S, Durigon R, Pearce SF, Rorbach J, He J, Brea-Calvo G, Minczuk M, Reyes A, Holt IJ, Spinazzola A
PloS one 2014;9(4):e93597

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MRM2 and MRM3 are involved in biogenesis of the large subunit of the mitochondrial ribosome.

Rorbach J, Boesch P, Gammage PA, Nicholls TJ, Pearce SF, Patel D, Hauser A, Perocchi F, Minczuk M
Molecular biology of the cell 2014 Sep 1;25(17):2542-55

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The arginine methyltransferase NDUFAF7 is essential for complex I assembly and early vertebrate embryogenesis.

Zurita Rendón O, Silva Neiva L, Sasarman F, Shoubridge EA
Human molecular genetics 2014 Oct 1;23(19):5159-70

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MPV17L2 is required for ribosome assembly in mitochondria.

Dalla Rosa I, Durigon R, Pearce SF, Rorbach J, Hirst EM, Vidoni S, Reyes A, Brea-Calvo G, Minczuk M, Woellhaf MW, Herrmann JM, Huynen MA, Holt IJ, Spinazzola A
Nucleic acids research 2014 Jul;42(13):8500-15

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Interactions between peptidyl tRNA hydrolase homologs and the ribosomal release factor Mrf1 in S. pombe mitochondria.

Dujeancourt L, Richter R, Chrzanowska-Lightowlers ZM, Bonnefoy N, Herbert CJ
Mitochondrion 2013 Nov;13(6):871-80

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The mitochondrial RNA-binding protein GRSF1 localizes to RNA granules and is required for posttranscriptional mitochondrial gene expression.

Antonicka H, Sasarman F, Nishimura T, Paupe V, Shoubridge EA
Cell metabolism 2013 Mar 5;17(3):386-98

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Overexpression of MRPS18-2 in Cancer Cell Lines Results in Appearance of Multinucleated Cells.

Shevchuk Z, Yurchenko MY, Darekar SD, Holodnuka-Kholodnyuk I, Kashuba VI, Kashuba EV
Acta naturae 2013 Jan;5(1):85-9

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Transcription-independent role for human mitochondrial RNA polymerase in mitochondrial ribosome biogenesis.

Surovtseva YV, Shadel GS
Nucleic acids research 2013 Feb 1;41(4):2479-88

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Mutations in mitochondrial ribosomal protein MRPL12 leads to growth retardation, neurological deterioration and mitochondrial translation deficiency.

Serre V, Rozanska A, Beinat M, Chretien D, Boddaert N, Munnich A, Rötig A, Chrzanowska-Lightowlers ZM
Biochimica et biophysica acta 2013 Aug;1832(8):1304-12

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C7orf30 is necessary for biogenesis of the large subunit of the mitochondrial ribosome.

Rorbach J, Gammage PA, Minczuk M
Nucleic acids research 2012 May;40(9):4097-109

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C7orf30 specifically associates with the large subunit of the mitochondrial ribosome and is involved in translation.

Wanschers BF, Szklarczyk R, Pajak A, van den Brand MA, Gloerich J, Rodenburg RJ, Lightowlers RN, Nijtmans LG, Huynen MA
Nucleic acids research 2012 May;40(9):4040-51

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Mitochondrial ribosomal protein L12 selectively associates with human mitochondrial RNA polymerase to activate transcription.

Surovtseva YV, Shutt TE, Cotney J, Cimen H, Chen SY, Koc EC, Shadel GS
Proceedings of the National Academy of Sciences of the United States of America 2011 Nov 1;108(44):17921-6

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NOA1 is an essential GTPase required for mitochondrial protein synthesis.

Kolanczyk M, Pech M, Zemojtel T, Yamamoto H, Mikula I, Calvaruso MA, van den Brand M, Richter R, Fischer B, Ritz A, Kossler N, Thurisch B, Spoerle R, Smeitink J, Kornak U, Chan D, Vingron M, Martasek P, Lightowlers RN, Nijtmans L, Schuelke M, Nierhaus KH, Mundlos S
Molecular biology of the cell 2011 Jan 1;22(1):1-11

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Human ERAL1 is a mitochondrial RNA chaperone involved in the assembly of the 28S small mitochondrial ribosomal subunit.

Dennerlein S, Rozanska A, Wydro M, Chrzanowska-Lightowlers ZM, Lightowlers RN
The Biochemical journal 2010 Sep 15;430(3):551-8

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Targeting of the cytosolic poly(A) binding protein PABPC1 to mitochondria causes mitochondrial translation inhibition.

Wydro M, Bobrowicz A, Temperley RJ, Lightowlers RN, Chrzanowska-Lightowlers ZM
Nucleic acids research 2010 Jun;38(11):3732-42

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 16139-1-AP(MRPS18B antibody) at dilution of 1:500

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The MRPS18B antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human MRPS18B. This antibody recognizes human,mouse,rat antigen. The MRPS18B antibody has been validated for the following applications: ELISA, WB, IHC, IF analysis.

Appendix

16139-1-AP.pdf

Show more

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human breast cancer using 16139-1-AP(MRPS18B antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human breast cancer using 16139-1-AP(MRPS18B antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue