MA5-24752

antibody from Invitrogen Antibodies

Targeting: MYO18B BK125H2.1

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-24752

Provider

Invitrogen Antibodies

Product name

MYO18B Monoclonal Antibody (1683CT270.39.50)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

1683CT270.39.50

Vial size

200 µL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MYO18B in paraformaldehyde-fixed, paraffin-embedded human skeletal muscle tissue sections. Samples were incubated with MYO18B monoclonal antibody (Product # MA5-24752) using a dilution of 1:25 for 1 hours at 37°C followed by an undiluted biotinylated goat polyvalent antibody. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MYO18B in paraformaldehyde-fixed, paraffin-embedded human heart tissue sections. Samples were incubated with MYO18B monoclonal antibody (Product # MA5-24752) using a dilution of 1:25 for 1 hours at 37°C followed by an undiluted biotinylated goat polyvalent antibody. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of (overlay histogram) of MYO18B in A431 cells (green line). Samples were incubated with MYO18B monoclonal antibody (Product # MA5-24752) using a dilution of 1:25 dilution for 60 min at 37°C followed by Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1:200 dilution for 40 min at 37°C. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the primary antibody. Isotype control antibody (blue line) was mouse IgG2b (1 μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.