MA5-24912

antibody from Invitrogen Antibodies

Targeting: NEUROG1 AKA, bHLHa6, Math4C, NEUROD3, ngn1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

MA5-24912

Provider

Invitrogen Antibodies

Product name

Neurogenin 1 Monoclonal Antibody (OTI8G8)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

OTI8G8

Vial size

100 µL

Concentration

1.00 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation was performed on HEK293T cells overexpressing NEUROG1. Antigen-antibody complexes were formed by incubating the sample with 2 µg of NEUROG1 monoclonal antibody (Product # MA5-24912) antibody and 0.1 mg of goat anti-mouse conjugated magnetic beads overnight. The sample was washed extensively and probed with rabbit anti-DDK polyclonal antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NEUROG1 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with NEUROG1 (Product # MA5-24912) monoclonal antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NEUROG1 in HepG2, HeLa, HT29, A549, COS7, Jurkat, MDCK, PC12, MCF7 cells using 35 µg per lane. Samples were probed with NEUROG1 (Product # MA5-24912) monoclonal antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of NEUROG1 in HT29 cells. Cells were probed with a NEUROG1 monoclonal antibody (Product # MA5-24912).