- PA5-34461 - Invitrogen Antibodies CMPK2 antibody

Chen Y, Wu L, Shi M, Zeng D, Hu R, Wu X, Han S, He K, Xu H, Shao X, Ma R
Frontiers in immunology 2022;13:788556

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of rat lung tissue lysate using a CMPK2 polyclonal antibody (Product # PA5-34461) at 1 µg/mL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of CMPK2 in rat lung tissue lysate with CMPK2 Polyclonal Antibody (Product # PA5-34461) at 1 µg/mL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence of CMPK2 in human lung tissue with CMPK2 Polyclonal Antibody (Product # PA5-34461) at 20 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis showed positive expression of CMPK2 in spinal cord tissues of the model and EA groups. (A) Immunofluorescence labeling represented the number of CMPK2-positive cells (green fluorescence) at 1, 3, and 7 days after SCI, and the CMPK2-positive protein staining area is shown in green. (B) Quantitative analysis of CMPK2-positive cells. *p < 0.05 compared with the model group; one-way anOVA was used for comparison. N = 5 rats/group. (C) BBB scores for the model and EA groups. N = 8 rats/group. * p < 0.05 compared with the sham group. Data are presented as the mean +- SEM. EA, electroacupuncture; BBB, Basso-Beattie-Bresnahan.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: The expression of CMPK2 in rat spinal cords was detected using WB and IF. (A) Western blotting was used to analyze the protein expression of CMPK2 in the sham and model groups after SCI. Representative protein bands are shown for CMPK at 1, 3, 7, and 14 days after SCI (N = 5 rats/group). *p < 0.05; two-way ANOVA was used for comparison. (B) Quantitative analysis of CMPK2-positive cells. *p < 0.05 compared with the sham group; one-way anOVA was used for comparison. N = 5 rats/group. (C) CMPK2 was expressed around the central canal of the gray matter of the spinal cord at 1, 3, and 7 days after spinal cord injury. CMPK2-positive cells are represented by green fluorescence. DAPI (blue) co-staining was used to identify positive cells. Data are presented as the mean +- SEM. WB, Western blotting; IF, immunofluorescence; SCI, spinal cord injury.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Effect of EA on CMPK2 expression in spinal cords of rats following CMPK2 knockdown. (A) BBB scores of rats in the Sham, Model, M+EA, M+AAV CMPK2, and M+AAV NC groups. N = 8. *p < 0.05 compared with the model group. (B) Western blotting analysis of CMPK2 expression in spinal cord tissues of rats in the Sham, Model, M+EA, M+AAV CMPK2, and M+AAV NC groups. A representative Western blotting and semiquantitative analysis of CMPK2 are shown. N = 5 rats/group. * p < 0.05 compared with the model group; one-way anOVA was used for comparison. (C) Evaluation of CMPK2 gene expression using qPCR in the Sham, Model, M+EA, M+AAV CMPK2, and M+AAV NC groups 3 days after SCI. N = 5 rats/group. * p < 0.05 compared with the model group; one-way anOVA was used for comparison. (D) Quantitative analysis of CMPK2-positive cells. * p < 0.05 compared with the model group; one-way anOVA was used for comparison. N = 5 rats/group. (E) Immunofluorescence was used to detect the expression of CMPK2 around the central canal of the spinal gray matter of rats in the Sham, Model, M+EA, M+AAV CMPK2, and M+AAV NC groups. Number of CMPK2-positive cells (green fluorescence) 3 days after SCI. DAPI (blue) co-staining was used to identify positive cells. All data are expressed as the mean +- SEM. EA, electroacupuncture; BBB, Basso-Beattie-Bresnahan; SCI, spinal cord injury.

PA5-34461