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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- GTX116436 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX116436, RRID:AB_11163796
- Product name
- SSU72 antibody [N1C3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references A gene-specific role for the Ssu72 RNAPII CTD phosphatase in HIV-1 Tat transactivation.
Chen Y, Zhang L, Estarás C, Choi SH, Moreno L Jr, Karn J, Moresco JJ, Yates JR 3rd, Jones KA
Genes & development 2014 Oct 15;28(20):2261-75
Genes & development 2014 Oct 15;28(20):2261-75
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot experiment using SSU72 Antibody (GTX116436) at 1:90000 dilution to detect 100ng of 25kDa recombinant SSU72 protein antigen.
- Validation comment
- WB-Ag
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SSU72 antibody detects SSU72 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 12% SDS-PAGE, and the membrane was blotted with SSU72 antibody (GTX116436) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (50 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with SSU72 antibody [N1C3] (GTX116436) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SSU72 antibody [N1C3] detects SSU72 protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. SSU72 antibody [N1C3] (GTX116436) diluted at 1:500.
