ASC-044-200UL

antibody from Invitrogen Antibodies

Targeting: SCN4B LQT10

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ASC-044-200UL

Provider

Invitrogen Antibodies

Product name

SCN4B (NaV beta 4) (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

200 µL

Concentration

0.85 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat brain (lanes 1 and 5), rat cortex (lane 2 and 6), mouse brain (lanes 3 and 7) and SH-SY5Y (lanes 4 and 8) lysates: - 1-4. Anti-SCN4B (NaV beta 4) (extracellular) Antibody (#ASC-044), (1:800).5-8. Anti-SCN4B (NaV beta 4) (extracellular) Antibody , preincubated with SCN4B/Nav beta 4 (extracellular) Blocking Peptide (#BLP-SC044).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat brain (lanes 1 and 5), rat cortex (lane 2 and 6), mouse brain (lanes 3 and 7) and SH-SY5Y (lanes 4 and 8) lysates: - 1-4. Anti-SCN4B (NaV beta 4) (extracellular) Antibody (#ASC-044), (1:800).5-8. Anti-SCN4B (NaV beta 4) (extracellular) Antibody , preincubated with SCN4B/Nav beta 4 (extracellular) Blocking Peptide (#BLP-SC044).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of NaV beta 4 in rat PC12 cells - Cell surface detection of NaV beta 4 in intact living ratpheochromocytoma (PC12) cells. A. Extracellular staining of cells using Anti-SCN4B (NaV beta 4) (extracellular) Antibody (#ASC-044), (1:50) followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody (red). B. Live view of the cells.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of NaV beta 4 in rat DRG - Immunohistochemical staining of adult rat dorsal root ganglion (DRG) using Anti-SCN4B (NaV beta 4) (extracellular) Antibody (#ASC-044) followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody . A. NaV beta 4 labeling (red) appears in the cell bodies of the DRG neurons. B. Nuclear staining using DAPI as the counterstain (blue). C. Merged image of A and B.