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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-78556 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Neurogenin 2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: U87-MG, SK-N-SH, mouse embryonic midbrain
- Concentration
- 1.13 mg/mL
Submitted references Proneural genes define ground-state rules to regulate neurogenic patterning and cortical folding.
Han S, Okawa S, Wilkinson GA, Ghazale H, Adnani L, Dixit R, Tavares L, Faisal I, Brooks MJ, Cortay V, Zinyk D, Sivitilli A, Li S, Malik F, Ilnytskyy Y, Angarica VE, Gao J, Chinchalongporn V, Oproescu AM, Vasan L, Touahri Y, David LA, Raharjo E, Kim JW, Wu W, Rahmani W, Chan JA, Kovalchuk I, Attisano L, Kurrasch D, Dehay C, Swaroop A, Castro DS, Biernaskie J, Del Sol A, Schuurmans C
Neuron 2021 Sep 15;109(18):2847-2863.e11
Neuron 2021 Sep 15;109(18):2847-2863.e11
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Neurogenin 2 in whole cell lysate using 30 µg of protein. Samples were separated with 12% SDS-PAGE and incubated with Neurogenin 2 polyclonal antibody (Product # PA5-78556) using a dilution of 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Neurogenin 2 in mouse tissue using 50 µg of protein. Samples were separated with 12 % SDS-PAGE and incubated with Neurogenin 2 polyclonal antibody (Product # PA5-78556) using a dilution of 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neurogenin 2 Polyclonal Antibody detects Neurogenin 2 protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 12 % SDS-PAGE, and the membrane was blotted with Neurogenin 2 Polyclonal Antibody (Product # PA5-78556) diluted by 1:2,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neurogenin 2 Polyclonal Antibody detects Neurogenin 2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Neurogenin 2 Polyclonal Antibody (Product # PA5-78556) diluted by 1:2,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Neurogenin 2 was performed in DIV9 rat E18 primary hippocampal neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Neurogenin 2 Polyclonal Antibody (Product # PA5 78556) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neurogenin 2 Polyclonal Antibody detects Neurogenin 2 protein expression at nucleus by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: Neurogenin 2 protein stained by Neurogenin 2 Polyclonal Antibody (Product # PA5-78556) diluted at 1:250. Red: SOX2, stained by SOX2 antibody [GT1876] diluted at 1:250. Blue: Fluoroshield with DAPI .
