PA5-80851

antibody from Invitrogen Antibodies

Targeting: NEUROG2 Atoh4, bHLHa8, Math4A, ngn-2, NGN2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-80851

Provider

Invitrogen Antibodies

Product name

Neurogenin 2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Neurogenin 2 in Lane A: HepG2 Whole Cell lysate (30 µg), Lane B: SH-SY5Y Whole Cell lysate (30 µg). Samples were probed using a Neurogenin 2 Polyclonal Antibody (Product # PA5-80851) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:29 kDa. Observed band size:29 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Neurogenin 2 Polyclonal Antibody (Product # PA5-80851) at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate, Lane B: SH-SY5Y Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti- RabbitIgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 29 kDa. (We are unsure of the identity of these extra bands).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of Neurogenin 2 in HESS9 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Neurogenin 2 Polyclonal Antibody (Product # PA5-80851, 1:300) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neurogenin 2 Immunoprecipitation using: Lane A: 0.5 mg K562 Whole Cell Lysate 4 µL with Neurogenin 2 Polyclonal Antibody (Product # PA5-80851) and 15 µL of 50 % Protein G agarose. Primary antibody: Neurogenin 2 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 26 kDa.