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LearnPA5-37276
antibody from Invitrogen Antibodies
Targeting: SCN8A
CerIII, CIAT, MED, NaCh6, Nav1.6, PN4
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
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- Product number
- PA5-37276 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SCN8A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 225 kDa.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SCN8A in Lane 1: MCF-7 cell lysate, Lane 2: Raw264.7 cell lysate, Lane 3: H9C2 cell lysate. Samples were incubated with SCN8A polyclonal antibody (Product # PA5-37276) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Na+ CP type VIII alpha using Na+ CP type VIII alpha polyclonal antibody (Product # PA5-37276) at a dilution of 1:500. Lane 1: MCF-7 cell lysate, Lane 2: Raw264.7 cell lysate, Lane 3: H9C2 cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of SCN8A was performed using 70% confluent log phase IMR-32 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SCN8A Polyclonal Antibody (Product # PA5-37276) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing staining in plasma membrane. Panel e represents control cells with no primary antibody to assess the background. The images were captured at 60X magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of SCN8A was achieved by transfecting IMR-32 cells with SCN8A specific siRNA (Silencer® select Product # s12535, s12534). Immunofluorescence analysis was performed on IMR-32 cells (untransfected, panel a,d), transfected with non-specific scrambled siRNA (panels b,e) and transfected with SCN8A specific siRNA (panel c,f). Cells were fixed, permeabilized, and labelled with SCN8A Polyclonal Antibody (Product # PA5-37276, 1:100 dilution), followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Reduction of specific signal was observed upon siRNA mediated knockdown (panel c,f) confirming specificity of the antibody to SCN8A (green). The images were captured at 60X magnification.
