PA5-31746

antibody from Invitrogen Antibodies

Targeting: OXR1 TLDC3

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-31746

Provider

Invitrogen Antibodies

Product name

OXR1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: NT2D1, IMR32, U87-MG, MCF-7. Predicted reactivity: Mouse (90%), Rat (89%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Mapping the H(+) (V)-ATPase interactome: identification of proteins involved in trafficking, folding, assembly and phosphorylation.

Merkulova M, Păunescu TG, Azroyan A, Marshansky V, Breton S, Brown D
Scientific reports 2015 Oct 7;5:14827

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 Localization of DMXL1, DMXL2, NCOA7, OXR1 and WDR7 in proton secreting cells of mouse kidney by immunocytochemistry. DMXL1 (( A) , red) co-localizes with the A-subunit of V-ATPase (( B ), green) in intercalated cells (ICs, indicated with arrows) of wild type mouse inner medullary collecting duct. WDR7 (( D ), red) also co-localizes with the same V-ATPase subunit ( E ), green) in ICs of wild type mouse cortical collecting duct. OXR1 ( G ), red) and V-ATPase (( H ), green) co-localize in the apical pole of distal convoluted tubule cells of wild type mice. DMXL2 ( J ), red) and NCOA7 ( M ), red) are expressed predominantly in ICs of B1-EGFP transgenic mouse inner medullary collecting duct (ICs are green in panels ( K , N )). Note, that in the B1-EGFP transgenic mouse EGFP is not fused to B1 subunit, rather EGFP expression is driven by B1 promoter. Thus, while EGFP is expressed specifically in ICs, its localization in these cells is purely cytosolic and does not follow V-ATPase sub-cellular localization pattern. Merged images ( C,F,I,L,O ) are shown in the right column. Scale bar = 10 mum.