PA5-56181

antibody from Invitrogen Antibodies

Targeting: SEPTIN7 CDC10, CDC3, SEPT7, SEPT7A

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-56181

Provider

Invitrogen Antibodies

Product name

Septin-7 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: VNIIPLIAKA DTLTPEECQQ FKKQIMKEIQ EHKIKIYEFP ETDDEEENKL VKKIKDRLPL AVVGSNTIIE VNGKRVRGRQ YPW Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 100%.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Proteomic analysis of the effect of the polyphenol pentagalloyl glucose on proteins involved in neurodegenerative diseases in activated BV‑2 microglial cells.

Mendonca P, Taka E, Soliman KFA
Molecular medicine reports 2019 Aug;20(2):1736-1746

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of Septin-7 in human cell line U-2 OS shows positivity in nucleoli, plasma membrane & actin filaments. Samples were probed using a Septin-7 Polyclonal Antibody (Product # PA5-56181).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of Septin-7 in human cell line A-431 using Septin-7 Polyclonal Antibody (Product # PA5-56181) shows localization to nucleoli, plasma membrane & actin filaments.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Septin-7 in human cerebellum using a Septin-7 Polyclonal Antibody (Product # PA5-56181) shows moderate cytoplasmic positivity in Purkinje cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Septin-7 in human lymph node using Septin-7 Polyclonal Antibody (Product # PA5-56181) shows moderate cytoplasmic positivity in non-germinal center cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Septin-7 in human testis using Septin-7 Polyclonal Antibody (Product # PA5-56181) shows moderate positivity in cells in seminiferous ducts.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Septin-7 in human cerebral cortex, lymph node, skeletal muscle and testis using Septin-7 Polyclonal Antibody (Product # PA5-56181) (A) shows similar protein distribution across tissues to an independent Septin-7 Polyclonal Antibody (B).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Septin-7 in human fallopian tube using Septin-7 Polyclonal Antibody (Product # PA5-56181) shows strong cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Septin-7 in human cerebral cortex using Septin-7 Polyclonal Antibody (Product # PA5-56181) shows strong cytoplasmic positivity in neuropil.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Septin-7 in human skeletal muscle using Septin-7 Polyclonal Antibody (Product # PA5-56181) shows no positivity in myocytes as expected.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3. Effect of PGG on the expression of septin-7 as determined by western analysis with automated capillary electrophoresis. (A) Graph of the amount of chemiluminescence measured in the different treatments. (B) Bands representing the protein expression in control (DMSO), PGG (25 uM), LPS/IFNgamma, and pretreated cells (PGG + LPS/IFNgamma) after 24 h of treatment (n=3). PPG, polyphenol pentagalloyl glucose (1,2,3,4,6-penta-O-galloyl-beta-D-glucose); LPS, lipopolysaccharide; IFNgamma, interferon gamma.