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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- TA347231 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Jarid1c Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Jarid1c Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- KDM5C
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- not determined
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB using the antibody against Jarid1c (lane 2) and with the preimmune serum (lane 1) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 175 kDa); the marker (in kDa) is shown on the left. Image shows that the additional bands at approximately 55 and 80 kDa are also present in the preimmune serum.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody against Jarid1c. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:7,000.
- Validation comment
- ELISA
