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LearnA301-035A
antibody from Invitrogen Antibodies
Targeting: KDM5C
DXS1272E, JARID1C, MRX13, SMCX, XE169
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- A301-035A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- JARID1C Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human JARID1C by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from HeLa cells. Antibodies: Affinity purified rabbit anti-JARID1C antibody (Product # A301-035A; lot A301-035A-1) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human JARID1C by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-JARID1C antibody A301-035A used for WB at 0.04 µg/ml (A) and 0.1 µg/ml (B) and used for IP at 3 µg/mg lysate. JARID1C was also immunoprecipitated by rabbit anti-JARID1C antibody A301-034A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human JARID1C by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-JARID1C antibody (Product # A301-035A; lot A301-035A-1) used for IP at 3 µg/mg lysate. JARID1C was also immunoprecipitated by rabbit anti-JARID1C antibody (Product # A301-034A), which recognizes an upstream epitope. For blotting immunoprecipitated JARID1C, Product # A301-035A was used at 0.1 µg/mL. Detection: Chemiluminescence with exposure time of 30 seconds.
