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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- NB100-55327 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-55327, RRID:AB_838911
- Product name
- Rabbit Polyclonal SMCX Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMCX Antibody [NB100-55327] - Detection of Human and Mouse Jarid1C by Western Blot. Samples: Whole cell lysate (50, 15 ug) from HeLa and (50 ug) from 293T, Jurkat, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Jarid1C antibody NB100-55327 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMCX Antibody [NB100-55327] - Detection of Human JARID1C on HeLa whole cell lysate using NB100-55327. JARID1C was also immunoprecipitated by rabbit anti-JARID1C antibody NB100-55328.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: SMCX Antibody [NB100-55327] - Detection of human Jarid1C (SMCX) by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Jarid1C antibody NB100-55327 used for IP at 6 ug per reaction. Jarid1C was also immunoprecipitated by rabbit anti-Jarid1C antibody NB100-55328. For blotting immunoprecipitated Jarid1C, NB100-55327 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation Sequencing: SMCX Antibody [NB100-55327] - Localization of Jarid1C Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-Jarid1C antibody NB100-55327 and analyzed by DNA sequencing. The figure illustrates the peak distribution of Jarid1C binding within a 500 Kb region of chromosome 1 as detected using anti-Jarid1C antibody NB100-55327. ChIP-seq validation performed by Diogenode, Denville, NJ.
